{"product_id":"hifair-v-reverse-transcriptase-600-u-mu-l-glycerol-free-bhz20800077","title":"Hifair™ V Reverse Transcriptase (600 U\/μL, Glycerol free)","description":"\u003cdiv class=\"rte text--pull\"\u003e\n                            \u003cdiv class=\"top_tabc proDetail\" data-v-444b0c7e=\"\"\u003e\n\u003cdiv data-v-444b0c7e=\"\"\u003e\n\u003cdiv data-v-444b0c7e=\"\"\u003e\n\u003cp class=\"p\"\u003e\u003cspan\u003e\u003cspan\u003eHifair™\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e\u003cspan\u003eV Reverse Transcriptase is an updated version of Hieff™\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003cspan\u003eM-MLV (H-) Reverse Transcriptase obtained by genetic engineering technology. It has higher cDNA synthesis ability, thermal stability, and reaction temperature limit (up to 60°C) than Hieff™\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e\u003cspan\u003eM-MLV (H-) Reverse Transcriptase. The synthesized cDNA product is up to 10kb. Hifair™\u003c\/span\u003e\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003e\u003cspan\u003eV Reverse Transcriptase enhances the affinity of the templates and is suitable for reverse transcription of RNA templates with complex secondary structures or low-copy genes.\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003ch3 class=\"p\"\u003e\n\u003cb\u003e\u003cspan\u003eFeatures\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003cspan\u003e\u003cspan style=\"mso-list: Ignore;\"\u003e \u003c\/span\u003e\u003c\/span\u003e\n\u003c\/h3\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003c!--[endif]--\u003e\u003cspan\u003eOutstanding thermal resistance.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003c\/span\u003e\u003c!-- [if !supportLists]--\u003e\u003cspan\u003eSuitable for RT-PCR\/RT-qPCR applications.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eGlycerol-free, more suitable for lyophilization.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch4 class=\"MsoNormal\"\u003e\n\u003cb\u003e\u003cspan\u003e\u003cspan\u003ePerformance demonstration\u003c\/span\u003e\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h4\u003e\n\u003cp\u003e\u003c!-- [if !supportLists]--\u003e\u003c!--[endif]--\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003c!-- [if !supportLists]--\u003e\u003cspan\u003e\u003cspan style=\"mso-list: Ignore;\"\u003e1. \u003c\/span\u003e\u003c\/span\u003e\u003c!--[endif]--\u003e\u003cb\u003e\u003cspan\u003e\u003cspan\u003eCapable of withstanding a reaction temperature of 58°C, it is suitable for the reverse transcription of complex RNA templates\u003c\/span\u003e\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003cspan\u003e.\u003c\/span\u003e\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/1_8efbd3fb-699c-493b-9c24-d517121cfc61.png?v=1733473526\"\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003e\u003cspan\u003eFigure 1. The Reverse Transcriptase of Hifair™ \u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e \u003cspan\u003eV Reverse Transcriptase and the brand T* were incubated at 55℃ and 58℃ respectively for 30min to detect the residual enzyme activity. The results showed that Hifair™ \u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003cspan\u003eV Reverse Transcriptase could still maintain 70% activity after incubation at 58℃ for 30min, which was much higher than that of the brand T*.\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003e\u003cspan\u003e2. The detection rate of RT-qPCR was better than that of other brands\u003c\/span\u003e\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003cspan\u003e,\u003c\/span\u003e\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003cspan\u003eand t\u003c\/span\u003e\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003cspan\u003ehe detection range was 1 pg-1 μg\u003c\/span\u003e\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003cspan\u003e.\u003c\/span\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003ch4 class=\"MsoNormal\"\u003e\u003cbr\u003e\u003c\/h4\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cb\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/2_fc2124e9-d245-4653-93c6-1cfd9cab77bb.png?v=1733473545\"\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp class=\"MsoBodyText\"\u003e\u003cspan\u003e\u003cspan\u003eFigure 2. Using 1 pg-1 μg (7 gradients) total RNA of 293T cells as template, reverse transcription was performed using Hifair™ \u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e \u003cspan\u003eV one-step RT-gDNA digestion SuperMix for qPCR(Cat#11142), V*, T*. The obtained cDNA was quantitatively analyzed by qPCR. The results showed that the sensitivity of 11142ES could reach 1 pg, which was better than that of other brands.\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003e\u003cstrong\u003eFAQ\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp\u003eQ: What are the features of this product?\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003eA: M-MLV (H-) Reverse Transcriptase is produced by modifying the wild-type M-MLV reverse transcriptase gene and expressing and purifying it in Escherichia coli. It has no RNaseH activity and possesses the charact\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Yeasen Biotechnology","offers":[{"title":"3 KU","offer_id":53294149763437,"sku":"11301ES06","price":95.0,"currency_code":"USD","in_stock":true},{"title":"12 KU","offer_id":53294158676333,"sku":"11301ES12","price":315.0,"currency_code":"USD","in_stock":true},{"title":"120 KU","offer_id":53294158709101,"sku":"11301ES62","price":2375.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/1_d6036a34-a8d5-4def-8ad8-01bc7cf3b594.png?v=1778101062","url":"https:\/\/www.ebiohippo.com\/products\/hifair-v-reverse-transcriptase-600-u-mu-l-glycerol-free-bhz20800077","provider":"BioHippo","version":"1.0","type":"link"}