{"product_id":"hifair-v-reverse-transcriptase-bhz20800076","title":"Hifair™ V Reverse Transcriptase","description":"\u003cdiv class=\"top_tabc proDetail\"\u003e\n\u003cdiv\u003e\n\u003cp class=\"p\"\u003eHifair™ V Reverse Transcriptase is an updated version of Hieff™M-MLV (H-) Reverse Transcriptase obtained by genetic engineering technology. It has higher cDNA synthesis ability, thermal stability, and reaction temperature limit (up to 60°C) than Hieff™ M-MLV (H-) Reverse Transcriptase. The synthesized cDNA product is up to 10kb. Hifair™ V Reverse Transcriptase enhances the affinity of the templates and is suitable for reverse transcription of RNA templates with complex secondary structures or low-copy genes.\u003cspan\u003e\u003c\/span\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003ch3 class=\"p\"\u003e\u003cstrong\u003eFeatures\u003cspan style=\"mso-list: Ignore;\"\u003e \u003c\/span\u003e\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli class=\"MsoNormal\"\u003e\n\u003c!--[endif]--\u003e\u003cspan\u003eOutstanding thermal resistance.\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli class=\"MsoNormal\"\u003e\n\u003cspan\u003e\u003c\/span\u003e\u003c!-- [if !supportLists]--\u003e\u003cspan\u003eSuitable for RT-PCR\/RT-qPCR applications.\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli class=\"MsoNormal\"\u003e\n\u003c!--[endif]--\u003e\u003cspan\u003eAvailable in various forms including high and low concentrations, and glycerol-free, to meet diverse requirements.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 class=\"MsoNormal\"\u003e\n\u003cspan\u003e\u003cb\u003eComponents\u003c\/b\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/h3\u003e\n\u003ctable border=\"0\" cellspacing=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp align=\"center\" class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eComponents No.\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp align=\"center\" class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003eName\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp align=\"center\" class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003e11300ES92 (10000U)\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp align=\"center\" class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan\u003e11300ES93 (5×10000U)\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e11300-A\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eHifair™ V Reverse Transcriptase (200U\/μL)\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e50 μL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e5×50 μL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e11300-B\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e5×Hifair™ V Buffer\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e250 μL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e5×250 μL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3 class=\"MsoNormal\"\u003e\n\u003cstrong\u003ePerformance demonstration\u003c\/strong\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003cp\u003e\u003c!-- [if !supportLists]--\u003e\u003c!--[endif]--\u003e\u003c\/p\u003e\n\u003c!-- [if !supportLists]--\u003e\u003cstrong\u003e1. \u003c!--[endif]--\u003eCapable of withstanding a reaction temperature of 58°C, it is suitable for the reverse transcription of complex RNA templates.\u003c\/strong\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/1_8efbd3fb-699c-493b-9c24-d517121cfc61.png?v=1733473526\"\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp align=\"justify\" class=\"MsoNormal\"\u003eFigure 1. The Reverse Transcriptase of Hifair™  V Reverse Transcriptase and the brand T* were incubated at 55℃ and 58℃ respectively for 30min to detect the residual enzyme activity. The results showed that Hifair™ V Reverse Transcriptase could still maintain 70% activity after incubation at 58℃ for 30min, which was much higher than that of the brand T*.\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e2. The detection rate of RT-qPCR was better than that of other brands, and the detection range was 1 pg-1 μg.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cb\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/2_fc2124e9-d245-4653-93c6-1cfd9cab77bb.png?v=1733473545\"\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp class=\"MsoBodyText\"\u003eFigure 2. Using 1 pg-1 μg (7 gradients) total RNA of 293T cells as template, reverse transcription was performed using Hifair™  V one-step RT-gDNA digestion SuperMix for qPCR(Cat#11142), V*, T*. The obtained cDNA was quantitatively analyzed by qPCR. The results showed that the sensitivity of 11142ES could reach 1 pg, which was better than that of other brands.\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\u003cp\u003e\u003cstrong\u003eGlycerol Content:\u003c\/strong\u003e Contains Glycerol\u003c\/p\u003e","brand":"Yeasen Biotechnology","offers":[{"title":"10000 U","offer_id":53294151008621,"sku":"11300ES92","price":380.0,"currency_code":"USD","in_stock":true},{"title":"5×10000 U","offer_id":53294156120429,"sku":"11300ES93","price":1530.0,"currency_code":"USD","in_stock":true},{"title":"200000 U","offer_id":53294156153197,"sku":"11300ES98","price":5465.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/1_8efbd3fb-699c-493b-9c24-d517121cfc61.png?v=1778101060","url":"https:\/\/www.ebiohippo.com\/products\/hifair-v-reverse-transcriptase-bhz20800076","provider":"BioHippo","version":"1.0","type":"link"}