{"product_id":"hint1-antibody-bha17102999","title":"HINT1 Antibody","description":"\u003ch2\u003eOverview\u003c\/h2\u003e \u003cp\u003eHINT1 antibody supplied as a purified reagent for WB, FACS, IHC-P, IF in Human, Mouse, Rat samples. This product is a monoclonal (mouse origin) antibody (host: Mouse; isotype: IgG1, kappa) intended for research use only. The target is commonly annotated with cytoplasmic, nuclear localization context, which may inform staining patterns.\u003c\/p\u003e \u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e \u003cul\u003e   \u003cli\u003e\n\u003cstrong\u003eAntibody identity:\u003c\/strong\u003e Monoclonal (mouse origin); host Mouse; isotype IgG1, kappa; clone 1500CT836.13.93.\u003c\/li\u003e   \u003cli\u003e\n\u003cstrong\u003eFormat and purification:\u003c\/strong\u003e format: Purified; purity: Protein G affinity.\u003c\/li\u003e   \u003cli\u003e\n\u003cstrong\u003eSpecies reactivity (reported):\u003c\/strong\u003e Human, Mouse, Rat.\u003c\/li\u003e   \u003cli\u003e\n\u003cstrong\u003eApplications (listed):\u003c\/strong\u003e WB, FACS, IHC-P, IF.\u003c\/li\u003e   \u003cli\u003e\n\u003cstrong\u003eImmunogen \/ epitope context:\u003c\/strong\u003e Recombinant human protein was used as the immunogen for the HINT1 antibody..\u003c\/li\u003e   \u003cli\u003e\n\u003cstrong\u003eLocalization:\u003c\/strong\u003e Cytoplasmic, nuclear (annotation-level guidance; cell state and isoforms can shift patterns).\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003eThese attributes help you align the antibody with the biological question (target state, sample type, and readout) while keeping interpretation grounded in appropriate controls.\u003c\/p\u003e \u003ch2\u003eBiological background\u003c\/h2\u003e \u003cp\u003eHINT1 is the intended antigen for this primary antibody. Reported biological context includes: Hydrolyzes purine nucleotide phosphoramidates with a single phosphate group, including adenosine 5'monophosphoramidate (AMP-NH2), adenosine 5'monophosphomorpholidate (AMP-morpholidate) and guanosine 5'monophosphomorpholidate (GMP-morpholidate). Hydrolyzes lysyl-AMP (AMP-N-epsilon-(N-alpha-acetyl lysine methyl ester)) generated by lysine tRNA ligase, as well as Met-AMP, His- AMP and Asp-AMP, lysyl-GMP (GMP-N-epsilon-(N-alpha-acetyl lysine methyl ester)) and AMP-N-alanine methyl ester. Subcellular localization information (Cytoplasmic, nuclear) can be useful when interpreting IF\/ICC patterns and selecting compartment-enriched lysates for WB.\u003c\/p\u003e \u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e \u003cul\u003e   \u003cli\u003ePost-translational modification mapping: phosphorylation-site–resolved antibodies are used to connect signaling inputs to target activation states and downstream readouts.\u003c\/li\u003e   \u003cli\u003ePerturbation and chemical biology: acetylation\/deacetylation pathways are frequently interrogated with inhibitors and genetic perturbations to separate direct regulation from adaptive responses.\u003c\/li\u003e   \u003cli\u003eSignal-flow and turnover studies: researchers pair immunodetection with perturbations that modulate enzymatic activity or proteostasis to understand regulation, stability, and feedback.\u003c\/li\u003e \u003c\/ul\u003e \u003ch2\u003eCommon research applications\u003c\/h2\u003e \u003cul\u003e   \u003cli\u003eWestern blot (WB): compare relative abundance\/isoform patterns across conditions and sample types; band shifts may reflect processing or post-translational modification.\u003c\/li\u003e   \u003cli\u003eFACS: commonly used to measure relative target levels or localization changes in the context of the experimental question.\u003c\/li\u003e   \u003cli\u003eIHC-P: commonly used to measure relative target levels or localization changes in the context of the experimental question.\u003c\/li\u003e   \u003cli\u003eImmunofluorescence (IF): visualize localization and co-localization patterns in cells or tissues.\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003eAcross these readouts, differences in signal intensity, localization, or complex enrichment are typically interpreted alongside sample-matched controls and independent evidence to distinguish regulation from technical variation.\u003c\/p\u003e \u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e \u003cul\u003e   \u003cli\u003eIsoforms, cleavage products, or post-translational modifications can alter apparent molecular weight and subcellular distribution; interpret bands and staining patterns in the context of expected biology and sample preparation.\u003c\/li\u003e   \u003cli\u003eSpecies differences and epitope conservation may affect binding; use matched positive controls and orthogonal evidence when comparing across organisms.\u003c\/li\u003e   \u003cli\u003eControl concepts: include appropriate isotype and secondary-only controls (for imaging), and consider genetic perturbations (knockout\/knockdown\/overexpression) or independent antibodies targeting distinct epitopes to strengthen conclusions.\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003eEpitope context is defined by the immunogen description; when available, align this with known domains, PTM sites, or family homology to anticipate potential cross-reactivity patterns. As a monoclonal antibody, binding is driven by a single epitope, which can support consistent recognition but may be sensitive to epitope masking by PTMs or conformational changes.\u003c\/p\u003e \u003c!-- Sources (internal): - UniProtKB entry (P49773) — UniProt Consortium — https:\/\/www.uniprot.org\/uniprotkb\/P49773\/entry - NCBI Gene search (HINT1) — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=HINT1 - Ensembl search (HINT1) — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=HINT1 - PubMed search (HINT1) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=HINT1 - Reactome pathway search (HINT1) — Reactome — https:\/\/reactome.org\/content\/query?q=HINT1 --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"In 1X PBS, pH 7.4, with 0.09% sodium azide \/ 0.08 ml","offer_id":53043224445293,"sku":"F54529-0.08ML","price":205.0,"currency_code":"USD","in_stock":true},{"title":"In 1X PBS, pH 7.4, with 0.09% sodium azide \/ 0.4 ml","offer_id":53043628671341,"sku":"F54529-0.4ML","price":439.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_a88f694d-fd0a-4eb8-baba-cfc65bb3c511.jpg?v=1771934207","url":"https:\/\/www.ebiohippo.com\/products\/hint1-antibody-bha17102999","provider":"BioHippo","version":"1.0","type":"link"}