{"product_id":"histone-h2a-mono-methyl-k118-antibody-hist1h2ab-bha17136130","title":"Histone H2A (mono methyl K118) Antibody \/ HIST1H2AB","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eHistone H2A (mono methyl K118) Antibody \/ HIST1H2AB is a anti-H2A Rabbit antibody Recombinant Rabbit Monoclonal clone 31H85 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF) with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e H2A\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Recombinant Rabbit Monoclonal, clone 31H85, isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Liquid\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, ICC, IF\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eHistone H2A (mono methyl K118) antibody detects Histone H2A monomethylated at lysine 118, encoded by the HIST1H2AB gene. Histone H2A is a core nucleosomal protein that forms the nucleosome octamer with histones H2B, H3, and H4. Post translational modifications of histone H2A regulate chromatin structure and gene expression. Monomethylation at lysine 118 represents an important epigenetic mark associated with transcriptional regulation and genome stability. Histone H2A (mono methyl K118) antibody provides researchers with a powerful tool for epigenetic and chromatin research.\u003cbr\u003e\u003cbr\u003eHistone modifications such as acetylation, methylation, and phosphorylation function as signals that recruit or repel regulatory proteins. Research using Histone H2A (mono methyl K118) antibody has demonstrated that lysine 118 monomethylation influences transcriptional elongation and DNA damage repair. The modification occurs in the globular domain of histone H2A, suggesting a structural impact on nucleosome stability and interactions with regulatory proteins.\u003cbr\u003e\u003cbr\u003eIn development, lysine 118 methylation has been linked to regulation of gene expression programs during differentiation. Studies with Histone H2A (mono methyl K118) antibody have shown that abnormal regulation of this mark can disrupt normal transcriptional control, leading to developmental abnormalities. The modification is also dynamically regulated in response to cellular stress, indicating its importance in adaptation and signaling.\u003cbr\u003e\u003cbr\u003eDysregulation of histone H2A methylation, including lysine 118, has been implicated in cancer. Research using Histone H2A (mono methyl K118) antibody has revealed that changes in this modification alter chromatin accessibility and influence oncogene or tumor suppressor gene expression. Because epigenetic states are reversible, histone modifications such as H2A K118 methylation represent potential therapeutic targets in oncology.\u003cbr\u003e\u003cbr\u003eHistone H2A (mono methyl K118) antibody is applied in chromatin immunoprecipitation, western blotting, and immunofluorescence. Chromatin immunoprecipitation maps genome wide distribution of the modification, western blotting distinguishes specific modification states, and immunofluorescence reveals nuclear distribution patterns. These applications make Histone H2A (mono methyl K118) antibody essential for chromatin biology research.\u003cbr\u003e\u003cbr\u003eBy supplying validated Histone H2A (mono methyl K118) antibody reagents,\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=H2A - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=H2A - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=H2A - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/H2A - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=H2A+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg\/ml BSA \/ 100 ul","offer_id":53047320215917,"sku":"FY13228","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_a3b3f7e3-e356-44e9-a75d-e365b5190850.jpg?v=1772019412","url":"https:\/\/www.ebiohippo.com\/products\/histone-h2a-mono-methyl-k118-antibody-hist1h2ab-bha17136130","provider":"BioHippo","version":"1.0","type":"link"}