{"product_id":"histone-h2b-acetyl-k16-antibody-hist2h2be-bha17135606","title":"Histone H2B (acetyl K16) Antibody \/ HIST2H2BE","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eHistone H2B (acetyl K16) Antibody \/ HIST2H2BE is a anti-H2B Rabbit antibody Recombinant Rabbit Monoclonal clone 31H96 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF) with listed reactivity in Human, Mouse.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e H2B\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Recombinant Rabbit Monoclonal, clone 31H96, isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Liquid\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, ICC, IF\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eHistone H2B (acetyl K16) antibody detects histone H2B specifically when acetylated at lysine 16. Histone H2B is one of the four core histone proteins that package DNA into nucleosomes, the fundamental units of chromatin. Acetylation of lysine residues within histone tails is a key epigenetic modification that influences chromatin structure and gene expression. Acetylation at lysine 16 of H2B is associated with transcriptional activation, chromatin remodeling, and regulation of DNA damage responses.\u003cbr\u003e\u003cbr\u003eHistone H2B (acetyl K16) antibody is widely applied in epigenetics, chromatin biology, and cancer research. By detecting acetylation at this specific site, researchers can examine how histone modifications regulate transcription and genome stability. This modification has been linked to gene activation during cell cycle progression, developmental processes, and stress responses.\u003cbr\u003e\u003cbr\u003e Chromatin immunoprecipitation with Histone H2B (acetyl K16) antibody isolates genomic regions enriched in this modification, providing insight into transcriptional regulation. Western blotting detects acetylated H2B protein, while immunofluorescence highlights nuclear localization and distribution of acetylated histones. These methods allow comprehensive analysis of chromatin modifications in diverse contexts.\u003cbr\u003e\u003cbr\u003eHistone acetylation at lysine 16 plays a role in DNA repair, particularly homologous recombination, by opening chromatin structure at sites of damage. Dysregulation of acetylation is implicated in oncogenesis, neurodegeneration, and developmental disorders. Histone H2B (acetyl K16) antibody supports studies investigating how epigenetic regulation contributes to disease and therapeutic strategies targeting histone modifiers.\u003cbr\u003e\u003cbr\u003eEpigenetic therapies, including histone deacetylase inhibitors, influence acetylation levels at lysine 16 and other residues. By applying this antibody, scientists can evaluate how drugs alter chromatin structure and gene expression. This provides both mechanistic and translational value for cancer and neurological disease research.\u003cbr\u003e\u003cbr\u003eHistone H2B (acetyl K16) antibody from\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=H2B - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=H2B - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=H2B - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/H2B - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=H2B+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg\/ml BSA \/ 100 ul","offer_id":53047297900909,"sku":"FY12703","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_89ffd8b0-6c9c-4528-8dbe-185e03ac19c2.jpg?v=1772019332","url":"https:\/\/www.ebiohippo.com\/products\/histone-h2b-acetyl-k16-antibody-hist2h2be-bha17135606","provider":"BioHippo","version":"1.0","type":"link"}