{"product_id":"histone-h2b-crotonyl-k12-antibody-hist2h2be-bha17135629","title":"Histone H2B (crotonyl K12) Antibody \/ HIST2H2BE","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eHistone H2B (crotonyl K12) Antibody \/ HIST2H2BE is a anti-H2B Rabbit antibody Recombinant Rabbit Monoclonal clone 31H73 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF) with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e H2B\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Recombinant Rabbit Monoclonal, clone 31H73, isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Liquid\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, ICC, IF\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eHistone H2B (crotonyl K12) antibody detects histone H2B specifically when modified by crotonylation at lysine 12. Histone H2B is encoded by multiple genes including HIST2H2BE, HIST1H2BB, and HIST1H2BC, with common identifiers such as H2B type 2E and H2B histone family members. Histone H2B is one of the four core histones that assemble into nucleosomes, which package and regulate DNA. Crotonylation is a recently discovered lysine acylation modification linked to transcriptional activation and open chromatin states. Modification at lysine 12 of H2B provides a dynamic marker of gene regulation in specific contexts such as development and stress responses.\u003cbr\u003e\u003cbr\u003eHistone H2B (crotonyl K12) antibody is widely applied in epigenetics, chromatin biology, and cancer research. By detecting crotonylation at this specific residue, researchers can examine how histone modifications extend beyond acetylation and methylation to influence transcription. Crotonylation at K12 has been associated with gene activation in embryonic stem cells, immune regulation, and cellular stress adaptation.\u003cbr\u003e\u003cbr\u003e ChIP with Histone H2B (crotonyl K12) antibody identifies promoter and enhancer regions enriched in this mark. Western blotting distinguishes crotonylated H2B from unmodified protein, while immunofluorescence highlights nuclear distribution of modified nucleosomes. These applications provide robust methods for exploring the biological relevance of histone crotonylation.\u003cbr\u003e\u003cbr\u003eHistone crotonylation is regulated by histone acetyltransferases and deacetylases, many of which also act on acetylation. This overlap suggests coordination between acylation states in regulating gene activity. Dysregulation of crotonylation has been implicated in cancer, neurodegeneration, and metabolic disease. Histone H2B (crotonyl K12) antibody supports studies into how this modification contributes to disease pathology and therapeutic opportunities.\u003cbr\u003e\u003cbr\u003eHistone H2B (crotonyl K12) antibody from\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=H2B - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=H2B - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=H2B - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/H2B - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=H2B+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg\/ml BSA \/ 100 ul","offer_id":53047298064749,"sku":"FY12726","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_ba433d63-737d-4e56-95e2-899fa6f61b15.jpg?v=1772019335","url":"https:\/\/www.ebiohippo.com\/products\/histone-h2b-crotonyl-k12-antibody-hist2h2be-bha17135629","provider":"BioHippo","version":"1.0","type":"link"}