{"product_id":"histone-h3-acetyl-k36-antibody-hist1h3a-bha17135927","title":"Histone H3 (acetyl K36) Antibody \/ HIST1H3A","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eHistone H3 (acetyl K36) Antibody \/ HIST1H3A is a anti-H3 Rabbit antibody Recombinant Rabbit Monoclonal clone 31H90 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF) with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e H3\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Recombinant Rabbit Monoclonal, clone 31H90, isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Liquid\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, ICC, IF\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eHistone H3 (acetyl K36) antibody detects acetylated Histone H3 at lysine 36, encoded by the HIST1H3A gene. Histone H3 is one of the core histone proteins forming the nucleosome, the basic structural unit of chromatin. Post-translational modifications of histones, including acetylation, phosphorylation, and methylation, regulate chromatin accessibility and gene expression. Acetylation at lysine 36 is associated with transcriptional activation, elongation, and chromatin organization. Histone H3 (acetyl K36) antibody provides a specific reagent to study this modification and its role in epigenetic regulation.\u003cbr\u003e\u003cbr\u003eHistone acetylation neutralizes positive charges on lysine residues, weakening histone-DNA interactions and making chromatin more accessible to transcription factors. Acetylation of lysine 36 in Histone H3 has been shown to facilitate RNA polymerase II elongation and promote recruitment of chromatin remodeling complexes. Studies with Histone H3 (acetyl K36) antibody have revealed that this modification marks actively transcribed genes and is enriched in gene bodies, distinguishing it from promoter-associated acetylations. This underscores its importance in transcriptional fidelity and elongation control.\u003cbr\u003e\u003cbr\u003eDysregulation of Histone H3 acetylation is linked to cancer, developmental disorders, and neurodegeneration. Altered acetylation patterns at lysine 36 contribute to abnormal gene expression in cancers such as leukemia and glioblastoma. Research using Histone H3 (acetyl K36) antibody has shown that histone acetyltransferases and deacetylases targeting this site are often misregulated in tumors, providing potential therapeutic targets. In neurobiology, reduced acetylation has been linked to impaired memory and synaptic plasticity, highlighting its role in cognition and brain function.\u003cbr\u003e\u003cbr\u003eHistone H3 (acetyl K36) antibody is applied in chromatin immunoprecipitation, western blotting, and immunofluorescence. Chromatin immunoprecipitation identifies genomic regions marked by acetylation, providing insights into gene regulation. Western blotting confirms acetylation status in whole-cell lysates, while immunofluorescence visualizes global distribution patterns in nuclei. These applications make the antibody essential for epigenetic and transcriptional research.\u003cbr\u003e\u003cbr\u003eBy supplying validated Histone H3 (acetyl K36) antibody reagents,\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=H3 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=H3 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=H3 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/H3 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=H3+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg\/ml BSA \/ 100 ul","offer_id":53047309959533,"sku":"FY13025","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_5c912942-345f-46a0-b335-7f28cd97a03f.jpg?v=1772019379","url":"https:\/\/www.ebiohippo.com\/products\/histone-h3-acetyl-k36-antibody-hist1h3a-bha17135927","provider":"BioHippo","version":"1.0","type":"link"}