{"product_id":"histone-h3-acetyl-k9-antibody-hist1h3a-bha17136077","title":"Histone H3 (acetyl K9) Antibody \/ HIST1H3A","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eHistone H3 (acetyl K9) Antibody \/ HIST1H3A is a anti-H3 Rabbit antibody Recombinant Rabbit Monoclonal clone 32H23 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow cytometry (FACS) with listed reactivity in Human, Mouse, Rat. Reported localization: Nuclear.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e H3\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Recombinant Rabbit Monoclonal, clone 32H23, isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Liquid\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, ICC, IF, IP, FACS\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eHistone H3 (acetyl K9) antibody detects Histone H3 acetylated at lysine 9, encoded by the HIST1H3A gene. Histone H3 is a core component of the nucleosome, which packages DNA into chromatin and regulates access to genetic information. Post-translational modifications such as acetylation, methylation, and phosphorylation determine chromatin structure and gene expression. Acetylation at lysine 9 is associated with active chromatin and transcriptional activation. Histone H3 (acetyl K9) antibody provides researchers with a powerful tool for studying epigenetic regulation and gene expression control.\u003cbr\u003e\u003cbr\u003eHistone H3 acetylated at lysine 9 plays an essential role in loosening chromatin structure, allowing transcription factors and polymerases to access DNA. Research using Histone H3 (acetyl K9) antibody has demonstrated that acetylation is mediated by histone acetyltransferases and removed by histone deacetylases. This modification serves as a signal for recruitment of chromatin remodeling complexes and transcriptional coactivators. The dynamic balance of acetylation and deacetylation at lysine 9 influences gene expression programs in development, differentiation, and disease.\u003cbr\u003e\u003cbr\u003eDysregulation of Histone H3 acetylation at lysine 9 has been implicated in cancer, neurological disorders, and inflammatory disease. Studies with Histone H3 (acetyl K9) antibody have shown that aberrant acetylation patterns are linked to oncogene activation or tumor suppressor silencing. In neurobiology, reduced acetylation contributes to cognitive decline, while histone deacetylase inhibitors restore acetylation and improve memory in experimental models. These findings underscore the therapeutic potential of targeting histone acetylation in disease.\u003cbr\u003e\u003cbr\u003eHistone modifications such as H3 acetyl K9 are also important in stem cell biology and reprogramming. Research using Histone H3 (acetyl K9) antibody has confirmed that acetylation levels influence pluripotency and lineage commitment. Elevated acetylation is associated with transcriptionally open chromatin, supporting reprogramming of somatic cells to induced pluripotent stem cells. These applications highlight the broad importance of this modification across biological systems.\u003cbr\u003e\u003cbr\u003eHistone H3 (acetyl K9) antibody is widely used in chromatin immunoprecipitation, western blotting, and immunofluorescence. Chromatin immunoprecipitation allows mapping of acetylation across genomes, western blotting detects modification-specific forms of histone H3, and immunofluorescence reveals distribution of acetylated chromatin in cells. These methods make Histone H3 (acetyl K9) antibody indispensable in epigenetics research.\u003cbr\u003e\u003cbr\u003eBy supplying validated Histone H3 (acetyl K9) antibody reagents,\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=H3 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=H3 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=H3 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/H3 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=H3+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg\/ml BSA \/ 100 ul","offer_id":53047314022765,"sku":"FY13175","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_607fc7df-b0a6-4ee7-a9e3-c6c2bb40b1b6.jpg?v=1772019422","url":"https:\/\/www.ebiohippo.com\/products\/histone-h3-acetyl-k9-antibody-hist1h3a-bha17136077","provider":"BioHippo","version":"1.0","type":"link"}