{"product_id":"histone-h3-antibody-hist1h3a-b-c-d-e-f-g-h-i-j-bha17135940","title":"Histone H3 Antibody \/ HIST1H3A\/B\/C\/D\/E\/F\/G\/H\/I\/J","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eHistone H3 Antibody \/ HIST1H3A\/B\/C\/D\/E\/F\/G\/H\/I\/J is a anti-H3 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as ELISA, Flow cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Western blot (WB) with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e H3\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e ELISA, FACS, IF, IHC, ICC, WB\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eHistone H3 antibody detects core histone protein H3, one of the fundamental structural components of chromatin that forms nucleosomes in eukaryotic cells. The UniProt recommended name is Histone H3, encoded by multiple human genes including HIST1H3A through HIST1H3J. Histone H3 plays a central role in DNA packaging, gene regulation, and epigenetic control through post-translational modifications that influence chromatin structure and transcriptional activity.\u003cbr\u003e\u003cbr\u003eFunctionally, Histone H3 antibody identifies a 136-amino-acid nuclear protein that forms an octameric core complex with histones H2A, H2B, and H4. This complex organizes 147 base pairs of DNA into nucleosomes, the fundamental repeating unit of chromatin. Modifications of histone H3, including methylation, acetylation, phosphorylation, and ubiquitination, act as epigenetic marks that determine chromatin accessibility and gene expression status. The N-terminal tail of H3 serves as the primary site for these covalent modifications.\u003cbr\u003e\u003cbr\u003eThe HIST1H3 family of genes is clustered on chromosome 6p22.2, encoding nearly identical variants of histone H3 proteins that differ slightly in sequence and expression timing. Histone H3 variants, including H3.1, H3.2, and H3.3, exhibit distinct deposition patterns; replication-dependent variants incorporate during DNA synthesis, while H3.3 is incorporated independently of replication to maintain transcriptionally active chromatin. These variants play unique roles in genome stability, DNA repair, and transcriptional memory.\u003cbr\u003e\u003cbr\u003eEpigenetically, histone H3 modifications define specific chromatin states: trimethylation at lysine 4 (H3K4me3) marks active promoters, while methylation at lysine 9 (H3K9me3) and lysine 27 (H3K27me3) signify repressed or heterochromatic regions. Phosphorylation of serine 10 (H3S10ph) correlates with mitotic chromatin condensation. Dysregulation of these modifications leads to altered gene expression and contributes to developmental disorders, cancer, and epigenetic diseases.\u003cbr\u003e\u003cbr\u003eHistone H3 antibody is widely used in epigenetics, chromatin biology, and transcriptional regulation research. It is suitable for western blotting, chromatin immunoprecipitation, immunofluorescence, and immunohistochemistry to detect histone H3 and its modified forms. This antibody supports studies of chromatin structure, gene expression, and nucleosome dynamics. It also serves as a normalization control in nuclear extract analyses due to its consistent expression across cell types.\u003cbr\u003e\u003cbr\u003eStructurally, histone H3 adopts a histone fold domain that facilitates DNA wrapping and histone-histone interactions within the nucleosome. Its flexible N-terminal tail extends from the nucleosome surface and interacts with chromatin modifiers and transcriptional machinery.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=H3 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=H3 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=H3 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/H3 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=H3+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047309861229,"sku":"FY13038","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_17c3e158-955b-4291-a297-d86a98fa66ef.jpg?v=1772019424","url":"https:\/\/www.ebiohippo.com\/products\/histone-h3-antibody-hist1h3a-b-c-d-e-f-g-h-i-j-bha17135940","provider":"BioHippo","version":"1.0","type":"link"}