{"product_id":"histone-h3-di-methyl-k79-antibody-hist1h3a-bha17135417","title":"Histone H3 (di-methyl K79) Antibody \/ HIST1H3A","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eHistone H3 (di-methyl K79) Antibody \/ HIST1H3A is a anti-H3 Rabbit antibody Recombinant Rabbit Monoclonal clone 31H84 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF) with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e H3\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Recombinant Rabbit Monoclonal, clone 31H84, isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Liquid\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, ICC, IF\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eHistone H3 (di-methyl K79) antibody detects histone H3 dimethylated at lysine 79, a post translational modification on the core histone H3 protein encoded by the HIST1H3A gene. Histone modifications regulate chromatin structure and gene expression by altering nucleosome dynamics and recruiting effector proteins. Dimethylation at lysine 79, located within the globular domain of histone H3, is catalyzed by the DOT1L methyltransferase and is associated with active transcription.\u003cbr\u003e\u003cbr\u003eHistone H3 (di-methyl K79) antibody is widely used in epigenetics, cancer research, and developmental biology. Detection of H3K79me2 serves as a marker of euchromatin and transcriptionally active regions. By applying this antibody, researchers can study how histone methylation influences chromatin remodeling and gene regulation.\u003cbr\u003e\u003cbr\u003eIn ChIP assays, Histone H3 (di-methyl K79) antibody enriches for genomic regions with active transcription. Western blot detects histone H3 with specific dimethylation at K79, while immunofluorescence maps nuclear distribution of this epigenetic modification. These applications allow detailed study of gene expression and epigenetic control.\u003cbr\u003e\u003cbr\u003eAberrant H3K79 methylation is implicated in leukemogenesis and other cancers. Fusion proteins such as MLL AF9 recruit DOT1L, leading to abnormal dimethylation and deregulated gene expression. By detecting H3K79me2 with specific antibodies, scientists can investigate mechanisms of oncogenic transformation and therapeutic responses to DOT1L inhibitors.\u003cbr\u003e\u003cbr\u003eBeyond cancer, H3K79 methylation regulates developmental programs, DNA damage repair, and cell cycle transitions. Its role in lineage specification makes it a critical marker in stem cell biology. Histone H3 (di-methyl K79) antibody therefore provides a versatile tool for exploring transcriptional regulation in both normal and pathological contexts.\u003cbr\u003e\u003cbr\u003eHistone H3 (di-methyl K79) antibody from\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=H3 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=H3 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=H3 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/H3 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=H3+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg\/ml BSA \/ 100 ul","offer_id":53047291543917,"sku":"FY12514","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_03c80a90-87c2-43aa-a0e6-309d48bb0d2a.jpg?v=1772019303","url":"https:\/\/www.ebiohippo.com\/products\/histone-h3-di-methyl-k79-antibody-hist1h3a-bha17135417","provider":"BioHippo","version":"1.0","type":"link"}