{"product_id":"histone-h3-symmetric-di-methyl-r17-antibody-hist1h3a-bha17135623","title":"Histone H3 (symmetric di methyl R17) Antibody \/ HIST1H3A","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eHistone H3 (symmetric di methyl R17) Antibody \/ HIST1H3A is a anti-H3 Rabbit antibody Recombinant Rabbit Monoclonal clone 31H83 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF) with listed reactivity in Human, Mouse.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e H3\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Recombinant Rabbit Monoclonal, clone 31H83, isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Liquid\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ICC, IF\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eHistone H3 (symmetric di methyl R17) antibody detects histone H3 modified at arginine 17 by symmetric dimethylation. Histone H3 is encoded by multiple genes including HIST1H3A, HIST1H3B, and HIST2H3A. Common identifiers include H3C1, H3F3, and histone family member H3. Histone H3 is a core nucleosomal protein that packages DNA into chromatin. Methylation at arginine 17 is catalyzed by protein arginine methyltransferases such as PRMT5 and influences transcriptional regulation and chromatin organization.\u003cbr\u003e\u003cbr\u003eHistone H3 (symmetric di methyl R17) antibody is widely applied in epigenetics, developmental biology, and cancer research. Symmetric dimethylation at arginine 17 is generally associated with gene repression, acting through recruitment of repressive complexes and alteration of nucleosome interactions. By detecting this modification, researchers can investigate how arginine methylation shapes chromatin function.\u003cbr\u003e\u003cbr\u003e ChIP with Histone H3 (symmetric di methyl R17) antibody identifies genomic regions enriched in this modification, revealing transcriptional silencing patterns. Western blotting distinguishes the modified form of histone H3, while immunofluorescence highlights nuclear localization and distribution within heterochromatin domains. These assays support detailed mapping of epigenetic landscapes.\u003cbr\u003e\u003cbr\u003eDysregulation of arginine methylation has been implicated in cancer, where altered PRMT5 activity promotes tumor growth and resistance to therapy. Histone H3 (symmetric di methyl R17) antibody therefore supports research into therapeutic targeting of arginine methyltransferases. The modification is also involved in stem cell regulation, X chromosome inactivation, and immune responses, broadening its biological significance.\u003cbr\u003e\u003cbr\u003eHistone H3 (symmetric di methyl R17) antibody from\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=H3 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=H3 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=H3 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/H3 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=H3+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg\/ml BSA \/ 100 ul","offer_id":53047297376621,"sku":"FY12720","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_4530ca99-eb23-4a3c-aee9-b1bcf3ddfc08.jpg?v=1772019332","url":"https:\/\/www.ebiohippo.com\/products\/histone-h3-symmetric-di-methyl-r17-antibody-hist1h3a-bha17135623","provider":"BioHippo","version":"1.0","type":"link"}