{"product_id":"histone-h3-tri-methyl-k27-antibody-hist1h3a-bha17135148","title":"Histone H3 (tri methyl K27) antibody \/ HIST1H3A","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eHistone H3 (tri methyl K27) antibody \/ HIST1H3A is an antibody targeting \u003cstrong\u003eHistone\u003c\/strong\u003e. Host: \u003cstrong\u003eRabbit\u003c\/strong\u003e. Monoclonal. Clone: \u003cstrong\u003e32H02\u003c\/strong\u003e. Format: Liquid. Applications: WB, IHC, ICC, IF.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e Histone.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunogen:\u003c\/strong\u003e A synthesized peptide derived from human Histone H3 (tri methyl K27) was used as the immunogen for the Histone H3 (tri….\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSpecies reactivity:\u003c\/strong\u003e Human, Mouse.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eConjugate:\u003c\/strong\u003e Unconjugated (no label listed).\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background (from provided description)\u003c\/h2\u003e\u003cp\u003eHistone H3 (tri methyl K27) antibody detects histone H3 trimethylated at lysine 27 (H3K27me3), a hallmark of transcriptional repression and epigenetic silencing. Histone H3, encoded in part by HIST1H3A, is a core component of nucleosomes that package DNA into chromatin.\u003c\/p\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWB:\u003c\/strong\u003e relative abundance and band pattern assessment using appropriate controls.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eIHC:\u003c\/strong\u003e tissue distribution assessment; interpret with matched controls.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eIF\/ICC:\u003c\/strong\u003e subcellular localization imaging with proper background controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eAccount for isoforms, paralogs, and post-translational modifications when interpreting signal.\u003c\/li\u003e\n\u003cli\u003eSupport specificity with biological controls (e.g., KO\/KD where available) and technical controls (e.g., secondary-only\/isotype).\u003c\/li\u003e\n\u003c\/ul\u003e\u003c!-- Sources (internal): - UniProt Knowledgebase (UniProt) – https:\/\/www.uniprot.org\/ - NCBI Gene (NIH\/NLM) – https:\/\/www.ncbi.nlm.nih.gov\/gene\/ - ZFIN – https:\/\/zfin.org\/ - Ensembl – https:\/\/www.ensembl.org\/ - Nature Methods (2015): A proposal for validation of antibodies – https:\/\/www.nature.com\/articles\/nmeth.3230 --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg\/ml BSA \/ 100 ul","offer_id":53046719971693,"sku":"FY12245","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_35a2b154-ad8f-46d0-8739-bceeaf54366d.jpg?v=1772010444","url":"https:\/\/www.ebiohippo.com\/products\/histone-h3-tri-methyl-k27-antibody-hist1h3a-bha17135148","provider":"BioHippo","version":"1.0","type":"link"}