{"product_id":"histone-h4-antibody-h4c1-2-3-4-5-6-8-9-11-12-13-14-15-16-bha17135330","title":"Histone H4 Antibody \/ H4C1\/2\/3\/4\/5\/6\/8\/9\/11\/12\/13\/14\/15\/16","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eHistone H4 Antibody \/ H4C1\/2\/3\/4\/5\/6\/8\/9\/11\/12\/13\/14\/15\/16 is a anti-H4 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e H4\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, IF, ICC\/IF, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eThe Histone H4 antibody targets Histone H4, one of the four core histones encoded by multiple HIST1H4 genes. Histone H4 forms part of the nucleosome core along with histones H2A, H2B, and H3, serving as a fundamental structural unit of chromatin. Each nucleosome consists of an octamer of histones wrapped by approximately 147 base pairs of DNA, compacting the genome into higher-order chromatin structures. The Histone H4 antibody provides researchers with a reliable tool to investigate chromatin organization, gene regulation, and post-translational histone modifications.\u003cbr\u003e\u003cbr\u003eHistone H4 plays a central role in chromatin stability and accessibility. Its N-terminal tail contains lysine residues that undergo multiple modifications including acetylation, methylation, and phosphorylation, which influence transcriptional activation or repression. The Histone H4 antibody allows detection of both native and modified forms, supporting studies into epigenetic regulation and DNA-templated processes. These modifications are dynamic and essential for replication, repair, and recombination.\u003cbr\u003e\u003cbr\u003eDuring DNA replication, Histone H4 is synthesized and assembled onto nascent DNA to restore chromatin structure. The Histone H4 antibody enables visualization of chromatin assembly and nucleosome spacing in proliferating cells. Dysregulation of H4 expression or modification patterns is associated with aberrant chromatin compaction and altered gene expression, contributing to cancer and developmental disorders. Histone H4 lysine 16 acetylation, for instance, plays a crucial role in chromatin decondensation and transcriptional activation.\u003cbr\u003e\u003cbr\u003eHistone H4 interacts with chromatin remodelers, histone chaperones, and modifying enzymes that control nucleosome positioning and dynamics. The Histone H4 antibody is widely used to study histone-protein interactions and to map modification patterns across the genome through chromatin immunoprecipitation (ChIP) assays. Changes in H4 modification landscapes serve as biomarkers for epigenetic reprogramming in response to stress, differentiation, or oncogenic transformation.\u003cbr\u003e\u003cbr\u003eThe Histone H4 antibody performs effectively in western blotting, immunofluorescence, and immunohistochemistry, producing strong nuclear staining characteristic of chromatin localization.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=H4 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=H4 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=H4 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/H4 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=H4+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047287808365,"sku":"FY12427","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_ea4587ec-5fbc-44ff-9c11-fe420589117d.jpg?v=1782237003","url":"https:\/\/www.ebiohippo.com\/products\/histone-h4-antibody-h4c1-2-3-4-5-6-8-9-11-12-13-14-15-16-bha17135330","provider":"BioHippo","version":"1.0","type":"link"}