{"product_id":"histone-h4-mono-methyl-k20-antibody-hist1h4a-bha17136144","title":"Histone H4 (mono methyl K20) Antibody \/ HIST1H4A","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eHistone H4 (mono methyl K20) Antibody \/ HIST1H4A is a anti-H4 Rabbit antibody Recombinant Rabbit Monoclonal clone 31H81 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF) with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e H4\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Recombinant Rabbit Monoclonal, clone 31H81, isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Liquid\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, ICC, IF\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eHistone H4 (mono methyl K20) antibody detects Histone H4 monomethylated at lysine 20, encoded by the HIST1H4A gene. Histone H4 is a core nucleosomal protein that organizes chromatin and regulates access to DNA. Post translational modifications of Histone H4, including acetylation and methylation, govern gene expression and genome stability. Monomethylation at lysine 20 is a widely studied epigenetic modification associated with transcriptional activation and DNA damage response. Histone H4 (mono methyl K20) antibody provides researchers with a specific tool for investigating chromatin regulation and epigenetic control of transcription.\u003cbr\u003e\u003cbr\u003eHistone H4 monomethylated at lysine 20 is catalyzed by the histone methyltransferase SETD8. Research using Histone H4 (mono methyl K20) antibody has shown that this mark is enriched at active gene promoters and regulatory regions, where it contributes to transcriptional competence. Unlike trimethylation at the same residue, which is associated with repression and heterochromatin formation, monomethylation correlates with active chromatin states. This makes the modification a critical epigenetic signal in fine tuning transcription.\u003cbr\u003e\u003cbr\u003eIn DNA damage response, Histone H4 K20 methylation recruits repair proteins to damaged sites, ensuring accurate repair of double strand breaks. Studies with Histone H4 (mono methyl K20) antibody have revealed that defects in this modification compromise genome stability and increase sensitivity to genotoxic stress. This underscores the functional significance of H4 K20 monomethylation in maintaining genome integrity.\u003cbr\u003e\u003cbr\u003eDysregulation of Histone H4 K20 methylation has been linked to cancer and developmental disorders. Research using Histone H4 (mono methyl K20) antibody has demonstrated that aberrant methylation patterns alter chromatin accessibility and transcriptional programs, promoting oncogenesis. Loss of SETD8 activity or abnormal regulation of this pathway has been associated with impaired differentiation and developmental abnormalities.\u003cbr\u003e\u003cbr\u003eHistone H4 (mono methyl K20) antibody is used in chromatin immunoprecipitation, western blotting, and immunofluorescence. Chromatin immunoprecipitation maps distribution of this modification across the genome, western blotting distinguishes between modified and unmodified forms of Histone H4, and immunofluorescence visualizes nuclear distribution of H4 K20 monomethylation. These experimental approaches make the antibody valuable for studying transcriptional regulation and DNA repair.\u003cbr\u003e\u003cbr\u003eBy supplying validated Histone H4 (mono methyl K20) antibody reagents,\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=H4 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=H4 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=H4 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/H4 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=H4+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg\/ml BSA \/ 100 ul","offer_id":53047319462253,"sku":"FY13242","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_39b7b0e5-9593-43e3-8a1a-038589b55076.jpg?v=1772019415","url":"https:\/\/www.ebiohippo.com\/products\/histone-h4-mono-methyl-k20-antibody-hist1h4a-bha17136144","provider":"BioHippo","version":"1.0","type":"link"}