{"product_id":"hmc-1-3-mast-cell-line-clone-1h9-bhc10923400","title":"HMC-1.3 Mast Cell Line (Clone 1H9) ; \u003cspan style=\"color: #ef6331;\"\u003eNot capable of histamine release (degranulation)\u003c\/span\u003e","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eA human mast cell line was derived from HMC-1.2 cells, which express constitutively active KIT due to the presence of two oncogenic mutations: D816V and V560G. Using CRISPR technology, a site-specific mutation of V560G generated a subline, HMC-1.3, harboring a single mono-allelic D816V-KIT variant.Although HMC-1.2 and HMC-1.3 cells exhibited comparable growth in vitro, HMC-1.3 displayed significantly reduced proliferation and tumorigenicity in vivo.\u003c\/p\u003e\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eModel identity:\u003c\/strong\u003e HMC-1.3 Mast Cell Line (Clone 1H9) ; \u003cspan style=\"color: #ef6331;\"\u003eNot capable of histamine release (degranulation)\u003c\/span\u003e is supplied as a tumor cell line derived from Human peripheral blood.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth properties:\u003c\/strong\u003e Semi-adherent\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth conditions:\u003c\/strong\u003e PriCoat™ T25 Flasks (G299) are recommended for optimal cell culture. Iscove’s Modified Dulbecco’s Medium (IMDM) (TM505) + 10% FBS(Regular*) + 2 mM L-Glutamine (G275) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂. *Do not heat-inactivate\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eProduct format:\u003c\/strong\u003e Frozen, BSL-2\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eThis cell-based model is generally used in immunology, hematology, and signaling studies. Donor\/background information is available for contextual interpretation.\u003c\/p\u003e\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003ePhosphorylation levels of ERK1\/2, AKT, and STAT5—key pathways associated with oncogenic KIT signaling—were similar between both cell lines. However, HMC-1.3 cells showed increased sensitivity to various pharmacological inhibitors, including clinically used tyrosine kinase inhibitors (TKIs) for advanced systemic mastocytosis, as well as JAK2 and BCL2 inhibitors.These findings indicate that the additional V560G-KIT mutation in HMC-1.2 cells modulates transcriptional programs driven by D816V-KIT, conferring a survival advantage, altered drug sensitivity, and enhanced tumorigenicity. This suggests that engineered human mast cells with a single D816V-KIT mutation (HMC-1.3) may serve as an improved preclinical model for mastocytosis research and drug testing.Note: HMC do not degranulate to IgE due to the lack of the receptor. Donor\/background information provided for this product: Male, 52, Mast cell leukemia.\u003c\/p\u003e\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eCell-line models continue to be used for tumor phenotype comparison, pathway perturbation studies, and assay development in controlled in vitro systems.\u003c\/li\u003e\n\u003cli\u003eEngineered and subtype-defined tumor lines are often used to compare growth behavior, reporter output, and response patterns across matched experimental conditions.\u003c\/li\u003e\n\u003cli\u003eWhen metastatic or lineage features are described, investigators commonly interpret results alongside morphology, passage history, and culture environment.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eCancer biology studies that compare proliferation-associated behavior, morphology, and pathway responses in vitro.\u003c\/li\u003e\n\u003cli\u003eAssay development for treatment response, reporter monitoring, or phenotype comparison under matched culture conditions.\u003c\/li\u003e\n\u003cli\u003eSide-by-side comparison of engineered versus parental background characteristics when relevant to the study design.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eChanges in morphology, growth rate, viability, or reporter signal are typically interpreted together with passage history, culture matrix, and the specified growth conditions for the model.\u003c\/p\u003e\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eMorphology, doubling behavior, and reporter or marker output can shift with passage number, substrate choice, and medium composition; these variables should be recorded alongside experimental readouts.\u003c\/li\u003e\n\u003cli\u003eMatched controls such as parental cells, untreated cultures, or parallel cultures maintained under identical conditions help distinguish background effects from biology of interest.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCulture and product details\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth Conditions:\u003c\/strong\u003e PriCoat™ T25 Flasks (G299) are recommended for optimal cell culture. Iscove’s Modified Dulbecco’s Medium (IMDM) (TM505) + 10% FBS(Regular*) + 2 mM L-Glutamine (G275) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂. *Do not heat-inactivate\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSplit Ratio:\u003c\/strong\u003e 1:10-1:20\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53180557525357,"sku":"T8388","price":2317.5,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/5hTp30dAKkVdBzJDBXcCBffIhhcrYW7Pksw5If5u.png?v=1774957894","url":"https:\/\/www.ebiohippo.com\/products\/hmc-1-3-mast-cell-line-clone-1h9-bhc10923400","provider":"BioHippo","version":"1.0","type":"link"}