{"product_id":"hmgcl-antibody-hydroxymethylglutaryl-coa-lyase-bha17136246","title":"HMGCL Antibody \/ Hydroxymethylglutaryl CoA lyase","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eHMGCL Antibody \/ Hydroxymethylglutaryl CoA lyase is a anti-HMGCL Rabbit antibody Recombinant Rabbit Monoclonal clone 30H50 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC) with listed reactivity in Human.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e HMGCL\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Recombinant Rabbit Monoclonal, clone 30H50, isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Liquid\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eHMGCL antibody detects Hydroxymethylglutaryl CoA lyase, encoded by the HMGCL gene. Hydroxymethylglutaryl CoA lyase is a mitochondrial enzyme involved in ketogenesis and leucine catabolism. HMGCL antibody provides researchers with a critical reagent to study energy metabolism, amino acid degradation, and inherited metabolic disorders.\u003cbr\u003e\u003cbr\u003eHydroxymethylglutaryl CoA lyase catalyzes the cleavage of HMG CoA into acetyl CoA and acetoacetate, a key step in ketone body production. Research using HMGCL antibody has shown that this enzyme provides alternative energy substrates during fasting, exercise, and carbohydrate restriction. By producing ketone bodies, Hydroxymethylglutaryl CoA lyase ensures energy supply for the brain, heart, and skeletal muscle when glucose availability is limited.\u003cbr\u003e\u003cbr\u003eStudies with HMGCL antibody have demonstrated that the enzyme is also essential for leucine degradation. Defects in HMGCL impair leucine catabolism and ketone body production, resulting in HMG CoA lyase deficiency, a rare autosomal recessive metabolic disorder. Patients present with recurrent metabolic crises, hypoglycemia, metabolic acidosis, and lethargy. These findings underscore the importance of Hydroxymethylglutaryl CoA lyase in both amino acid metabolism and energy balance.\u003cbr\u003e\u003cbr\u003eIn addition to inherited disease, HMGCL function is relevant in cancer and diabetes. Research using HMGCL antibody has shown that alterations in ketone body metabolism influence tumor growth and metabolic adaptation. Some cancers reprogram ketone metabolism to support proliferation, while others are inhibited by high ketone levels. In diabetes, dysregulation of ketogenesis contributes to ketoacidosis, making HMGCL a critical enzyme for clinical monitoring and research.\u003cbr\u003e\u003cbr\u003eHydroxymethylglutaryl CoA lyase is localized to the mitochondrial matrix. Studies with HMGCL antibody have confirmed mitochondrial enrichment, with expression highest in liver and kidney, the major ketogenic organs. Tissue distribution reflects its role in systemic energy metabolism and adaptation to nutrient availability.\u003cbr\u003e\u003cbr\u003eHMGCL antibody is widely applied in western blotting, immunohistochemistry, and enzymatic activity studies. Western blotting quantifies protein expression, immunohistochemistry identifies tissue distribution, and activity assays measure enzymatic function. These applications make HMGCL antibody indispensable for metabolic biology research.\u003cbr\u003e\u003cbr\u003eBy providing validated HMGCL antibody reagents,\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=HMGCL - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=HMGCL - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=HMGCL - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/HMGCL - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=HMGCL+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg\/ml BSA \/ 100 ul","offer_id":53047323754861,"sku":"FY13344","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_119398b9-d58a-4207-9395-fdd0e6ee2da1.jpg?v=1772019431","url":"https:\/\/www.ebiohippo.com\/products\/hmgcl-antibody-hydroxymethylglutaryl-coa-lyase-bha17136246","provider":"BioHippo","version":"1.0","type":"link"}