{"product_id":"hn080-cell-line-bhc10901995","title":"HN080 Cell Line","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eThis tumor cell line is cultured from patient tumor tissue; cells were passaged 30 times to confirm immortalization. Cell characterization was conducted through STR of the patient tumor and cell line.\u003c\/p\u003e\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eModel identity:\u003c\/strong\u003e HN080 Cell Line is supplied as a tumor cell line derived from Human head\/neck.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth properties:\u003c\/strong\u003e Adherent, epithelial\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth conditions:\u003c\/strong\u003e Use of PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. PriGrow II (TM002) + 5% FBS + 1% NEAA + 1 mM Sodium Pyruvate + 10 mM HEPES + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂. Cells are sensitive to freeze\/thaw cycles; supplementing media with 20% FBS is recommended during the initial thaw. Once cells recover, 5% FBS is sufficient.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eProduct format:\u003c\/strong\u003e Frozen, BSL-2\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eThis cell-based model is generally used in head\/neck biology, phenotype comparison, and assay development studies. Donor\/background information is available for contextual interpretation.\u003c\/p\u003e\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eThis model supports studies in head\/neck biology, phenotype comparison, and assay development. It can be used to examine morphology, growth behavior, and experimental responses in cultured cells. Donor\/background information provided for this product: Female, Squamous Cell Carcinoma.\u003c\/p\u003e\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eCell-line models continue to be used for tumor phenotype comparison, pathway perturbation studies, and assay development in controlled in vitro systems.\u003c\/li\u003e\n\u003cli\u003eEngineered and subtype-defined tumor lines are often used to compare growth behavior, reporter output, and response patterns across matched experimental conditions.\u003c\/li\u003e\n\u003cli\u003eWhen metastatic or lineage features are described, investigators commonly interpret results alongside morphology, passage history, and culture environment.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eCancer biology studies that compare proliferation-associated behavior, morphology, and pathway responses in vitro.\u003c\/li\u003e\n\u003cli\u003eAssay development for treatment response, reporter monitoring, or phenotype comparison under matched culture conditions.\u003c\/li\u003e\n\u003cli\u003eSide-by-side comparison of engineered versus parental background characteristics when relevant to the study design.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eChanges in morphology, growth rate, viability, or reporter signal are typically interpreted together with passage history, culture matrix, and the specified growth conditions for the model.\u003c\/p\u003e\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eMorphology, doubling behavior, and reporter or marker output can shift with passage number, substrate choice, and medium composition; these variables should be recorded alongside experimental readouts.\u003c\/li\u003e\n\u003cli\u003eMatched controls such as parental cells, untreated cultures, or parallel cultures maintained under identical conditions help distinguish background effects from biology of interest.\u003c\/li\u003e\n\u003cli\u003eSTR profiling information reported for this product: Details Amelogenin : X,X CSF1PO : 10,10 D2S1338 : 16,24 D3S1358 : 17,17 D5S818 : 13,13 D7S820 : 8,8 D8S1179 : 12,15 D13S317 : 8,8 D16S539 : 8,8 D19S433 : 14,15.2 D21S11 : 30,32.2 FGA : 24,24 Penta D : 10,12 Penta E : 7,11 TH01 : 7,7 TPOX : 8,8 vWA : 14,14.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCulture and product details\u003c\/h2\u003e\n\u003cul\u003e\u003cli\u003e\n\u003cstrong\u003eGrowth Conditions:\u003c\/strong\u003e Use of PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. PriGrow II (TM002) + 5% FBS + 1% NEAA + 1 mM Sodium Pyruvate + 10 mM HEPES + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂. Cells are sensitive to freeze\/thaw cycles; supplementing media with 20% FBS is recommended during the initial thaw. Once cells recover, 5% FBS is sufficient.\u003c\/li\u003e\u003c\/ul\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53180536717677,"sku":"T8120","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/t8120.png?v=1774957847","url":"https:\/\/www.ebiohippo.com\/products\/hn080-cell-line-bhc10901995","provider":"BioHippo","version":"1.0","type":"link"}