{"product_id":"hot-start-taq-dna-polymerase-bhp15200308","title":"Hot Start Taq DNA Polymerase","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-qc-badge{display:inline-block;background:#e6f4ea;color:#1a7a3c;border:1px solid #a8d5b5;border-radius:4px;padding:6px 14px;font-size:14px;font-weight:600}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-license-box{background:#f5f5f5;border:1px solid #ddd;border-radius:4px;padding:12px 16px;font-size:14px;color:#444}.bhp-desc .bhp-regulatory-row{display:flex;gap:16px;flex-wrap:wrap;margin:6px 0 10px}.bhp-desc .bhp-reg-item{background:#f0f6ff;border:1px solid #ccd9f0;border-radius:4px;padding:6px 12px;font-size:14px}.bhp-desc .bhp-reg-item strong{color:#003366;display:block;font-size:12px;text-transform:uppercase;letter-spacing:.5px}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}.bhp-desc .bhp-comp-table th{width:auto}\u003c\/style\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eDNA Polymerases\u003c\/span\u003e\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eHot Start Taq DNA Polymerase is an enzyme solution in which a monoclonal antibody against Taq enzyme and Taq enzyme are mixed in a 1:1 activity unit. Taq enzyme antibody has a very high affinity with Taq enzyme. It can block the activity of Taq enzyme before high temperature denaturation, so it can inhibit primer dimer and non-specific amplification very effectively, greatly improving the accuracy of the PCR reaction. Hot Start Taq DNA Polymerase does not require special high temperature treatment. The pre-denaturation step in conventional PCR reaction conditions can start the activity of Taq enzyme. It is suitable for various Taq enzyme-based hot-start PCR and qPCR reactions. The PCR product has an A at the 3' end, which can be cloned directly with TA vector.\u003c\/p\u003e\n\u003cp\u003eThe enzyme is supplied at 5 U\/µL together with 10× PCR Buffer (Mg²⁺) and ddH₂O; no separate MgCl₂ is supplied. A 50 µL reaction uses 0.5–1.0 µL of polymerase at a final Mg²⁺ concentration of 2 mM, adjustable between 2 and 5 mM. Cycling is 94°C for 1–5 min, then 25–35 cycles of 94°C for 15 sec, 50–60°C for 30 sec and 72°C for 30 sec, with a final 72°C for 5 min.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eConcentration\u003c\/th\u003e\n\u003ctd\u003e5 U\/µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eUnit definition\u003c\/th\u003e\n\u003ctd\u003eIncorporates 10 nmol dNTPs into acid-insoluble material in 30 min at 74°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eHot start mechanism\u003c\/th\u003e\n\u003ctd\u003eMonoclonal antibody against Taq enzyme mixed with Taq enzyme in a 1:1 activity unit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eActivation\u003c\/th\u003e\n\u003ctd\u003eNo special high temperature treatment; activated by the pre-denaturation step of conventional PCR\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePurity\u003c\/th\u003e\n\u003ctd\u003eGreater than 99% by SDS-PAGE\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSupplied buffer\u003c\/th\u003e\n\u003ctd\u003e10× PCR Buffer (Mg²⁺)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTemplate input per 50 µL\u003c\/th\u003e\n\u003ctd\u003eHuman genomic DNA 0.05–0.5 µg; E. coli genomic DNA 10–100 ng; λ DNA 0.5–5 ng; plasmid DNA 0.1–10 ng\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eProduct ends\u003c\/th\u003e\n\u003ctd\u003eA at the 3' end for direct TA vector cloning\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eKit Components\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table bhp-comp-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eComponent\u003c\/th\u003e\n\u003cth\u003e500 U (100 µL)\u003c\/th\u003e\n\u003cth\u003e2500 U (500 µL)\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHot Start Taq DNA Polymerase (5 U\/µL)\u003c\/td\u003e\n\u003ctd\u003e100 µL\u003c\/td\u003e\n\u003ctd\u003e500 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e10× PCR Buffer (Mg²⁺)\u003c\/td\u003e\n\u003ctd\u003e1 mL\u003c\/td\u003e\n\u003ctd\u003e5 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eddH₂O\u003c\/td\u003e\n\u003ctd\u003e1 mL × 2\u003c\/td\u003e\n\u003ctd\u003e10 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eHot-start PCR\u003c\/li\u003e\n\u003cli\u003eTaq-based qPCR\u003c\/li\u003e\n\u003cli\u003eTA cloning of PCR products\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eStorage \u0026amp; Handling\u003c\/h2\u003e\n\u003cp\u003eAll components are stored at -20°C with a shelf life of two years.\u003c\/p\u003e\n\u003ch2\u003eUsage Notes\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eTemplate purity is critical; impure templates should not exceed one tenth of the reaction volume.\u003c\/li\u003e\n\u003cli\u003ePrimer working concentration is typically 0.2 µM.\u003c\/li\u003e\n\u003cli\u003eSet the annealing temperature about 5°C below the primer Tm.\u003c\/li\u003e\n\u003cli\u003eUse an extension time of about 1 kb\/min.\u003c\/li\u003e\n\u003cli\u003eExcessive cycle numbers increase non-specific products.\u003c\/li\u003e\n\u003cli\u003eThaw components and keep them on ice while assembling the reaction.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSafety \u0026amp; Regulatory\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\u003cp\u003eFor research use only. Not intended for clinical, diagnostic or therapeutic use. Follow the manufacturer's manual and standard laboratory safety practice.\u003c\/p\u003e\u003c\/div\u003e\n\u003cdiv class=\"bhp-regulatory-row\"\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eGrade\u003c\/strong\u003eResearch Use Only\u003c\/div\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eManufacturer\u003c\/strong\u003eELK Biotechnology\u003c\/div\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eCatalog No.\u003c\/strong\u003eEQ006\u003c\/div\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eBioHippo SKU\u003c\/strong\u003eBHP15200308\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"ELK Biotechnology","offers":[{"title":"2500 U (500 uL)","offer_id":53965434323309,"sku":"EQ006-2500U","price":442.0,"currency_code":"USD","in_stock":true},{"title":"500 U x 10","offer_id":53965434356077,"sku":"EQ006-500UX10","price":0.0,"currency_code":"USD","in_stock":true},{"title":"2500 U x 10","offer_id":53965434388845,"sku":"EQ006-2500UX10","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/EQ006-a-1.jpg?v=1789416775","url":"https:\/\/www.ebiohippo.com\/products\/hot-start-taq-dna-polymerase-bhp15200308","provider":"BioHippo","version":"1.0","type":"link"}