{"product_id":"hoxb1-reporter-lentivirus-bhv19400076","title":"HOXB1 Reporter Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eHOXB1 is a homeobox transcription factor that helps establish anterior-posterior patterning during embryonic development, with a particularly important role in specifying segmental identity in the developing hindbrain. HOXB1 expression is tightly controlled by promoter autoregulatory elements (ARE), which contain binding sites for MEIS1-PBX-HOX complexes that direct precise spatial and temporal gene expression. Through these cooperative interactions, HOXB1 contributes to the formation of rhombomeres and the patterning of cranial motor neurons. As a member of the HOX family of developmental regulators, HOXB1 is a valuable model for studying transcriptional control of body plan formation and neural development.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe HOXB1 Reporter Lentivirus places a reporter gene under the control of tandem HOXB1 DNA-binding elements derived from the HOXB1 promoter autoregulatory elements (ARE), providing a sensitive readout of HOXB1 transcriptional activity in human and mouse cells. Reporter options include fluorescent (GFP, EGFP, d2GFP, mCherry, RFP) and luminescent (firefly luciferase) formats, with blasticidin or puromycin selection for stable reporter cell line establishment.\u003c\/p\u003e\n\u003cp\u003eThe particles are purified by PEG precipitation and sucrose gradient centrifugation and efficiently transduce difficult-to-transfect cells, including primary and cryopreserved cultures. Detection is possible by fluorescence microscopy, flow cytometry, or luminometry. Applications include monitoring HOXB1 activity in developmental models and studying MEIS1-PBX-HOX-mediated transcription.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis reporter lentivirus places a d2GFP, EGFP, Firefly Luc, GFP, mCherry, RFP reporter gene under the control of tandem consensus response elements specific for the HOXB1 transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Puromycin) and\/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.\u003c\/p\u003e\n\u003cp\u003eStable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GFP \/ Puromycin \/ 5x10^6","offer_id":53251618472301,"sku":"LTV-0083-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6","offer_id":53310901059949,"sku":"LTV-0083-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Puromycin \/ 2x10^6","offer_id":53310901092717,"sku":"LTV-0083-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Blasticidin \/ 2x10^6","offer_id":53310901125485,"sku":"LTV-0083-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Blasticidin \/ 5x10^6","offer_id":53310901158253,"sku":"LTV-0083-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Puromycin \/ 5x10^6","offer_id":53310901191021,"sku":"LTV-0083-6S","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/HOXB1-Fig.-2.jpg?v=1776934149","url":"https:\/\/www.ebiohippo.com\/products\/hoxb1-reporter-lentivirus-bhv19400076","provider":"BioHippo","version":"1.0","type":"link"}