{"product_id":"hoxb6-antibody-homeobox-protein-hox-b6-bha17135604","title":"HOXB6 Antibody \/ Homeobox protein Hox-B6","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eHOXB6 Antibody \/ Homeobox protein Hox-B6 is a anti-HOXB6 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS) with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e HOXB6\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, FACS\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eHOXB6 antibody recognizes Homeobox protein Hox-B6, a sequence-specific transcription factor that directs developmental patterning and cell differentiation. Encoded by the HOXB6 gene on chromosome 17q21.32, this protein belongs to the homeobox (HOX) family of transcription factors that determine positional identity along the anterior-posterior axis during embryogenesis. HOXB6 contains a highly conserved homeodomain that binds specific DNA sequences to regulate expression of genes involved in morphogenesis, hematopoiesis, and organ development. It acts downstream of retinoic acid signaling and cooperates with cofactors such as PBX1 and MEIS1 to control target gene activation.\u003cbr\u003e\u003cbr\u003eDuring development, HOXB6 expression is restricted to specific regions of the embryo corresponding to the thoracic and lumbar domains. It contributes to the specification of vertebrae and rib patterning and influences limb and neural development. In adult tissues, HOXB6 expression is largely downregulated but can be reactivated in certain cell lineages, particularly within the hematopoietic system. Overexpression of HOXB6 has been observed in leukemias, where it drives proliferation and disrupts differentiation by altering transcriptional programs that control cell fate.\u003cbr\u003e\u003cbr\u003eThe HOXB6 antibody is a valuable reagent for developmental biology and cancer research. Western blot analysis detects a 25 kilodalton band corresponding to HOXB6, and immunohistochemical staining shows nuclear localization consistent with its role as a transcription factor. The antibody supports studies examining HOX gene regulation, retinoic acid signaling, and transcriptional networks controlling tissue differentiation. In experimental systems, HOXB6 expression is used as a marker of posterior HOX cluster activation, which defines segmental identity and developmental timing.\u003cbr\u003e\u003cbr\u003eFunctionally, HOXB6 binds to enhancer regions of genes that regulate cell cycle progression and differentiation, including MYC and CDKN1A. Dysregulation of HOXB6 is implicated in oncogenesis, particularly in acute myeloid leukemia, where it cooperates with MEIS1 to promote leukemic transformation. In solid tumors, aberrant HOXB6 expression correlates with invasive potential and epithelial-mesenchymal transition. The HOXB6 antibody facilitates detection of these expression changes, enabling characterization of HOX-mediated gene regulation in both normal and malignant cells.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=HOXB6 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=HOXB6 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=HOXB6 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/HOXB6 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=HOXB6+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047297737069,"sku":"FY12701","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_e55b2b58-251d-4c37-8783-51841af9d9d6.jpg?v=1782237030","url":"https:\/\/www.ebiohippo.com\/products\/hoxb6-antibody-homeobox-protein-hox-b6-bha17135604","provider":"BioHippo","version":"1.0","type":"link"}