{"product_id":"hpd-antibody-4hppd-4-hydroxyphenylpyruvate-dioxygenase-bha17136342","title":"HPD Antibody \/ 4HPPD \/ 4-hydroxyphenylpyruvate dioxygenase","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eHPD Antibody \/ 4HPPD \/ 4-hydroxyphenylpyruvate dioxygenase is a anti-HPD Rabbit antibody Polyclonal (rabbit origin) supplied in Antigen affinity purified format. Recommended for workflows such as Western blot (WB), IHC-P, Flow cytometry (FACS), Immunofluorescence (IF), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasmic, nuclear speckles.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e HPD\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Antigen affinity purified\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC-P, FACS, IF, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eHPD (4-hydroxyphenylpyruvate dioxygenase) is a key enzyme in the catabolism of the amino acid tyrosine. It catalyzes the conversion of 4-hydroxyphenylpyruvate to homogentisate, an essential step in the tyrosine degradation pathway. By facilitating this reaction, HPD plays an important role in energy metabolism and the production of downstream metabolites. A HPD antibody is widely used in studies of amino acid metabolism and inherited metabolic disorders.    \u003cbr\u003e\u003cbr\u003e  HPD is highly expressed in the liver and kidneys, organs that are central to amino acid catabolism. Its activity ensures that tyrosine is efficiently broken down, preventing the accumulation of toxic intermediates. Mutations in the HPD gene are associated with tyrosinemia type III, a rare metabolic disorder characterized by elevated levels of tyrosine in the blood and neurological symptoms. Researchers employ a HPD antibody to investigate enzyme function, tissue distribution, and the molecular basis of metabolic diseases.    \u003cbr\u003e\u003cbr\u003e  Beyond its role in metabolism, HPD has clinical importance as a therapeutic target. Small-molecule inhibitors of HPD are being explored to reduce toxic metabolite accumulation in tyrosinemia type I, where blocking this enzyme helps redirect metabolic flux. Using a HPD antibody supports translational research into such therapies by enabling detailed study of enzyme expression and regulation.    \u003cbr\u003e\u003cbr\u003e\n\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=HPD - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=HPD - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=HPD - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/HPD - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=HPD+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"0.5mg\/ml if reconstituted with 0.2ml sterile DI water \/ 100 ug","offer_id":53047326835053,"sku":"RQ8924","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_279a6c4b-0220-4f56-8b56-2408bf91c079.jpg?v=1772019449","url":"https:\/\/www.ebiohippo.com\/products\/hpd-antibody-4hppd-4-hydroxyphenylpyruvate-dioxygenase-bha17136342","provider":"BioHippo","version":"1.0","type":"link"}