{"product_id":"hrp-conjugated-protein-a-bha21012557","title":"HRP Conjugated Protein A","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eHRP Conjugated Protein A is an affinity reagent used to bind or detect antibody- and biotin-based reagents in common immunoassays.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eRecognition:\u003c\/strong\u003e Designed to recognize immunoglobulins from Protein A for secondary detection.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eConjugate\/label:\u003c\/strong\u003e HRP provides a defined detection chemistry (e.g., fluorophore, enzyme, or biotin–streptavidin systems).\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Concentrated, Liquid.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSpecificity notes (provided):\u003c\/strong\u003e This HRP conjugated Protein A. Human IgG1, IgG2 and IgG4 bind strongly,while IgG3 does not bind.There are also many instances in which monoclonal antibodies do not bind to Protein A, especially the majority of rat immunoglobulins and mouse IgG1..\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eHRP Conjugated Protein A\u003c\/p\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e \u003cp\u003eSecondary antibodies enable indirect detection by binding to the constant regions of primary antibodies. Fragment specificity matters: reagents labeled as (H+L) recognize epitopes on both heavy and light chains of the target-species immunoglobulin, while Fc- or light-chain-specific secondaries can reduce off-target signal in some multiplex or isotype-specific designs. Label choice (fluorophore, enzyme, biotin) affects sensitivity, multiplex compatibility, and how signal should be interpreted across sample types.\u003c\/p\u003e \u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e \u003cul\u003e \u003cli\u003eMultiplex imaging and spatial biology workflows increasingly rely on well-matched secondary antibodies to minimize cross-reactivity between species and isotypes.\u003c\/li\u003e \u003cli\u003eBiotin–streptavidin amplification remains common for tissue staining and low-abundance targets, with controls used to monitor endogenous biotin background where relevant.\u003c\/li\u003e \u003cli\u003eQuantitative immunoassays often combine consistent secondary reagents with standardized detection chemistries to support cross-experiment comparability.\u003c\/li\u003e \u003c\/ul\u003e \u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e converts primary antibody binding into a chemiluminescent or fluorescent signal for relative protein-level comparisons.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA\/plate assays:\u003c\/strong\u003e provides scalable detection in microplate formats for binding or titer-style measurements.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e \u003cul\u003e \u003cli\u003e\n\u003cstrong\u003eCross-reactivity and background:\u003c\/strong\u003e non-target binding can arise from endogenous immunoglobulins, closely related species, or multiplexed primary antibodies. Species selection and cross-adsorption (when specified) help reduce unwanted signal.\u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eControl concepts:\u003c\/strong\u003e no-primary controls, isotype controls, and orthogonal detection channels help distinguish specific staining from background. For biotin-based systems, consider controls for endogenous biotin where relevant.\u003c\/li\u003e \u003c\/ul\u003e \u003c!-- Sources (internal): - Understanding secondary antibodies – selection guide — Abcam — https:\/\/docs.abcam.com\/pdf\/secondary-antibodies\/secondary-antibodies-selection-guide.pdf - Choosing a secondary antibody: fragment specificity — Thermo Fisher Scientific — https:\/\/documents.thermofisher.com\/TFS-Assets\/LSG\/brochures\/TR0059-Choose-secondary-Ab.pdf - Cross-adsorbed secondary antibodies and cross-reactivity — Jackson ImmunoResearch — https:\/\/www.jacksonimmuno.com\/secondary-antibody-resource\/technical-tips\/cross-adsorbed-and-cross-reactivity\/ - What does (H+L) mean? — Biotium — https:\/\/biotium.com\/faqs\/what-does-hl-mean\/ - Comparison of antibody IgG-binding proteins — Thermo Fisher Scientific — https:\/\/www.thermofisher.com\/us\/en\/home\/life-science\/antibodies\/antibodies-learning-center\/antibodies-resource-library\/antibody-methods\/comparison-antibody-igg-binding-proteins.html - Avidin–biotin complex method for IHC detection — Thermo Fisher Scientific — https:\/\/www.thermofisher.com\/us\/en\/home\/life-science\/protein-biology\/protein-biology-learning-center\/protein-biology-resource-library\/pierce-protein-methods\/avidin-biotin-complex-method-ihc-detection.html --\u003e","brand":"Boster Bio","offers":[{"title":"0.5 ml","offer_id":53072632447341,"sku":"BA1080-0.5ml","price":135.0,"currency_code":"USD","in_stock":true},{"title":"1 ml","offer_id":53072634511725,"sku":"BA1080-1ml","price":280.0,"currency_code":"USD","in_stock":true}],"url":"https:\/\/www.ebiohippo.com\/products\/hrp-conjugated-protein-a-bha21012557","provider":"BioHippo","version":"1.0","type":"link"}