{"product_id":"hs6st1-stable-knockout-heparan-sulfate-cell-line-hs6st1-negative-bhc10922976","title":"Hs6st1 Stable Knockout Heparan Sulfate Cell Line (Hs6st1-\/-)","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eThis cell line (Cat. T3380) featues a stable knockout of HS 6-O-sulfotransferease-1 (Hs6st1) using Cre-Lox recombinase.\u003c\/p\u003e\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eModel identity:\u003c\/strong\u003e Hs6st1 Stable Knockout Heparan Sulfate Cell Line (Hs6st1-\/-) is supplied as an engineered cell line derived from Mouse lung.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth properties:\u003c\/strong\u003e Adherent, endothelial\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth conditions:\u003c\/strong\u003e PriCoat™ T25 Flasks (G299) coated with Gelatin Coating Solution (0.1%) (TM063) are required for optimal cell adhesion and growth. PriGrow III (TM003) + 10% FBS(Regular*) + 30µg\/mL Endothelial Cell Growth Supplement (TM130) + 50µg\/mL Heparin + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂. *Do not heat-inactivate\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eProduct format:\u003c\/strong\u003e Frozen, BSL-2\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eThis cell-based model is generally used in vascular biology, signaling, and assay development studies. Donor\/background information is available for contextual interpretation.\u003c\/p\u003e\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eThe control cell line for this catalog is T3379 - Hs6st1 Wild-Type Heparan Sulfate Stable Cell Line (Hs6st1f\/f). Target gene deletion was confirmed using genomic DNA genotyping. This cell line is part of a comprehensive heparan sulfate (HS)-mutant mouse lung endothelial cell (MLEC) library. These cell lines are suitable for investigations examining the roles and structures of HS within the cellular context. Complete list of mutant and wild-type heparan sulfate mouse lung endothelial cell lines from this panel: Cat. T3373 - Ndst1 Wild-Type Heparan Sulfate Stable Cell Line (Ndst1f\/f)Cat. T3374 - Ndst1 Stable Knockout Heparan Sulfate Cell Line (Ndst1-\/-)Cat. T3375 - Ndst1f\/f ;Ndst2-\/- Heparan Sulfate Stable Cell Line (Ndst1f\/f ;Ndst2-\/-)Cat. T3376 - Glce Stable Knockout Heparan Sulfate Cell Line (Glce-\/-)Cat. T3377 - Hs2st1 Wild-Type Heparan Sulfate Stable Cell Line (Hs2st1f\/f)Cat. T3378 - Hs2st1 Stable Knockout Heparan Sulfate Cell Line (Hs2st1-\/-)Cat. T3379 - Hs6st1 Wild-Type Heparan Sulfate Stable Cell Line (Hs6st1f\/f)Cat. T3380 - Hs6st1 Stable Knockout Heparan Sulfate Cell Line (Hs6st1-\/-)Cat. T3381 - Hs6st1f\/f;Hs6st2-\/- Heparan Sulfate Stable Cell Line (Hs6st1f\/f;Hs6st2-\/-)Cat. T3382 - Hs6st1;Hs6st2 Stable Knockout Heparan Sulfate Cell Line (Hs6st1-\/-;Hs6st2-\/-)Cat. T3383 - Hs3st1 Stable Knockout Heparan Sulfate Cell Line (Hs3st1-\/-)Cat. T3384 - Hs3st4 Stable Knockout Heparan Sulfate Cell Line (Hs3st4-\/-)Cat. T3385 - Hs3st1;Hsst4 Stable Knockout Heparan Sulfate Cell Line (Hs3st1-\/-;Hsst4-\/-)Cat. T3386 - Sulf1\/Sulf2 Wild-Type Heparan Sulfate Stable Cell Line (Sulf1f\/f;Sulf2f\/f)Cat. T3387 - Sulf1\/Sulf2 Stable Knockout Heparan Sulfate Cell Line (Sulf1-\/-;Sulf2-\/-)Cat. T3490 - Ndst1\/Ndst2 Stable Knockout Heparan Sulfate Cell Line (Ndst1-\/- ;Ndst2-\/-)Cat. T3491 - Ext1 Wild-Type Heparan Sulfate Stable Cell Line (Ext1f\/f)Cat. T3492 - Ext1 Stable Knockout Heparan Sulfate Cell Line (Ext1-\/-) Donor\/background information provided for this product: Adult C57BL6. Expression information reported for the model: Mouse HS 6-O-sulfotransferease-1 Knockout.\u003c\/p\u003e\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eEngineered cell lines are widely used for reporter-based readouts, perturbation studies, and assay optimization in reproducible culture systems.\u003c\/li\u003e\n\u003cli\u003eReporter or transgene-bearing models are often compared with matched parental or control cells to interpret signal changes in context.\u003c\/li\u003e\n\u003cli\u003eExpression trends are typically evaluated alongside passage number, selection pressure, and baseline growth behavior.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eRoutine expansion and maintenance of a defined cell model for downstream in vitro experiments.\u003c\/li\u003e\n\u003cli\u003ePhenotype, signaling, or marker-expression studies performed under standardized culture conditions.\u003c\/li\u003e\n\u003cli\u003eCell-based assay development in which passage number, growth surface, and medium composition are tracked as experimental variables.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eChanges in morphology, growth rate, viability, or reporter signal are typically interpreted together with passage history, culture matrix, and the specified growth conditions for the model.\u003c\/p\u003e\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eMorphology, doubling behavior, and reporter or marker output can shift with passage number, substrate choice, and medium composition; these variables should be recorded alongside experimental readouts.\u003c\/li\u003e\n\u003cli\u003eMatched controls such as parental cells, untreated cultures, or parallel cultures maintained under identical conditions help distinguish background effects from biology of interest.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCulture and product details\u003c\/h2\u003e\n\u003cul\u003e\u003cli\u003e\n\u003cstrong\u003eGrowth Conditions:\u003c\/strong\u003e PriCoat™ T25 Flasks (G299) coated with Gelatin Coating Solution (0.1%) (TM063) are required for optimal cell adhesion and growth. PriGrow III (TM003) + 10% FBS(Regular*) + 30µg\/mL Endothelial Cell Growth Supplement (TM130) + 50µg\/mL Heparin + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂. *Do not heat-inactivate\u003c\/li\u003e\u003c\/ul\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53180548088173,"sku":"T3380","price":494.4,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/y6lLRL3tPT6WYrXIiyOGvvXXqp34e1rNkUePKHtM.png?v=1774957873","url":"https:\/\/www.ebiohippo.com\/products\/hs6st1-stable-knockout-heparan-sulfate-cell-line-hs6st1-negative-bhc10922976","provider":"BioHippo","version":"1.0","type":"link"}