{"product_id":"hse-reporter-lentivirus-bhv19400008","title":"HSE Reporter Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe heat shock element (HSE) is a conserved promoter motif that mediates the heat shock response, the cell's protective program against proteotoxic stress. Heat, oxidative stress, and protein-damaging conditions activate heat shock factor 1 (HSF1), which trimerizes, accumulates in the nucleus, and binds HSEs to induce molecular chaperones such as HSP70 and HSP90. These chaperones refold or clear misfolded proteins and restore proteostasis. HSF1 also influences cell survival, metabolism, and stress adaptation, and its activity is frequently elevated in cancer to support malignant proteostasis. HSE-driven reporters provide a direct readout of HSF1 activation and heat shock response signaling.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe HSE Reporter Lentivirus places a reporter gene under the control of tandem heat shock elements, providing a sensitive readout of HSF1 activation and heat shock response pathway activity in human and mouse cells. Reporter options include fluorescent (GFP, RFP) and luminescent (firefly and Renilla luciferase) formats, with puromycin or blasticidin selection for rapid generation of stable reporter cell lines. Signal can be measured by fluorescence microscopy, flow cytometry, or luminometry.\u003c\/p\u003e\n\u003cp\u003eThe particles are purified by PEG precipitation and sucrose gradient centrifugation and efficiently transduce difficult-to-transfect cells, including primary and cryopreserved cultures. Applications include monitoring proteotoxic stress responses, studying proteostasis in cancer, and screening compounds that modulate HSF1 activity.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis reporter lentivirus places a Firefly Luc, Renilla Luc, GFP, RFP, Luc reporter gene under the control of tandem consensus response elements specific for the Heat shock response pathway (HSF-1) transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Puromycin, Blasticidin) and\/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.\u003c\/p\u003e\n\u003cp\u003eStable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GFP \/ Puromycin \/ 5x10^6","offer_id":53251619062125,"sku":"LTV-0009-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6","offer_id":53310891884909,"sku":"LTV-0009-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Puromycin \/ 2x10^6","offer_id":53310891917677,"sku":"LTV-0009-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Blasticidin \/ 2x10^6","offer_id":53310891950445,"sku":"LTV-0009-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Blasticidin \/ 5x10^6","offer_id":53310891983213,"sku":"LTV-0009-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Renilla Luc \/ Blasticidin \/ 2x10^6","offer_id":53310892015981,"sku":"LTV-0009-4SIC","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/TF-Reporter-Vector-circular_2c5d26ae-5462-4d2e-a1cc-4a37a55815fd.jpg?v=1776934271","url":"https:\/\/www.ebiohippo.com\/products\/hse-reporter-lentivirus-bhv19400008","provider":"BioHippo","version":"1.0","type":"link"}