{"product_id":"hsp60-antibody-hspd1-bha17104010","title":"HSP60 Antibody \/ HSPD1","description":"\u003ch2\u003eOverview\u003c\/h2\u003e \u003cp\u003eHSP60 antibody supplied as a antigen affinity purified reagent for WB, IHC-P, IF, ICC, FACS in Human, Mouse, Rat samples. This product is a polyclonal (rabbit origin) antibody (host: Rabbit; isotype: Rabbit IgG) intended for research use only. The target is commonly annotated with cytoplasm (mitochondria) localization context, which may inform staining patterns.\u003c\/p\u003e \u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e \u003cul\u003e   \u003cli\u003e\n\u003cstrong\u003eAntibody identity:\u003c\/strong\u003e Polyclonal (rabbit origin); host Rabbit; isotype Rabbit IgG.\u003c\/li\u003e   \u003cli\u003e\n\u003cstrong\u003eFormat and purification:\u003c\/strong\u003e format: Antigen affinity purified; purity: Antigen affinity.\u003c\/li\u003e   \u003cli\u003e\n\u003cstrong\u003eSpecies reactivity (reported):\u003c\/strong\u003e Human, Mouse, Rat.\u003c\/li\u003e   \u003cli\u003e\n\u003cstrong\u003eApplications (listed):\u003c\/strong\u003e WB, IHC-P, IF, ICC, FACS.\u003c\/li\u003e   \u003cli\u003e\n\u003cstrong\u003eImmunogen \/ epitope context:\u003c\/strong\u003e An amino acid sequence from the C-terminus of rat HSP60 (YDAMLGDFVNMVEK) was used as the immunogen for this HSP60 antibody..\u003c\/li\u003e   \u003cli\u003e\n\u003cstrong\u003eLocalization:\u003c\/strong\u003e Cytoplasm (mitochondria) (annotation-level guidance; cell state and isoforms can shift patterns).\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003eThese attributes help you align the antibody with the biological question (target state, sample type, and readout) while keeping interpretation grounded in appropriate controls.\u003c\/p\u003e \u003ch2\u003eBiological background\u003c\/h2\u003e \u003cp\u003eHSP60 is the intended antigen for this primary antibody. Reported biological context includes: Heat shock 60KD protein is a member of the chaperonin class of protein factors and the nuclear encoded mitochondrial HSP60 is required for the assembly into oligomeric complexes of proteins imported into the mitochondrial matrix. HSP60 is linked head to head comprising approximately 17 kb and consist of 12 exons. Subcellular localization information (Cytoplasm (mitochondria)) can be useful when interpreting IF\/ICC patterns and selecting compartment-enriched lysates for WB.\u003c\/p\u003e \u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e \u003cul\u003e   \u003cli\u003eSpatial and single-cell approaches: imaging-based and cytometry workflows increasingly quantify heterogeneity and relocalization rather than only bulk abundance.\u003c\/li\u003e   \u003cli\u003eInteraction-centric biology: IP-based enrichment and proteomics are widely used to define complexes, binding partners, and context-specific interactomes.\u003c\/li\u003e \u003c\/ul\u003e \u003ch2\u003eCommon research applications\u003c\/h2\u003e \u003cul\u003e   \u003cli\u003eWestern blot (WB): compare relative abundance\/isoform patterns across conditions and sample types; band shifts may reflect processing or post-translational modification.\u003c\/li\u003e   \u003cli\u003eIHC-P: commonly used to measure relative target levels or localization changes in the context of the experimental question.\u003c\/li\u003e   \u003cli\u003eImmunofluorescence (IF): visualize localization and co-localization patterns in cells or tissues.\u003c\/li\u003e   \u003cli\u003eImmunocytochemistry (ICC): visualize intracellular distribution and morphology-linked changes in cultured cells.\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003eAcross these readouts, differences in signal intensity, localization, or complex enrichment are typically interpreted alongside sample-matched controls and independent evidence to distinguish regulation from technical variation.\u003c\/p\u003e \u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e \u003cul\u003e   \u003cli\u003eIsoforms, cleavage products, or post-translational modifications can alter apparent molecular weight and subcellular distribution; interpret bands and staining patterns in the context of expected biology and sample preparation.\u003c\/li\u003e   \u003cli\u003eSpecies differences and epitope conservation may affect binding; use matched positive controls and orthogonal evidence when comparing across organisms.\u003c\/li\u003e   \u003cli\u003eControl concepts: include appropriate isotype and secondary-only controls (for imaging), and consider genetic perturbations (knockout\/knockdown\/overexpression) or independent antibodies targeting distinct epitopes to strengthen conclusions.\u003c\/li\u003e \u003c\/ul\u003e \u003cp\u003eEpitope context is defined by the immunogen description; when available, align this with known domains, PTM sites, or family homology to anticipate potential cross-reactivity patterns. As a polyclonal antibody, recognition spans multiple epitopes, which can improve detection across conformations but may broaden background depending on sample context.\u003c\/p\u003e \u003c!-- Sources (internal): - UniProtKB entry (P63039) — UniProt Consortium — https:\/\/www.uniprot.org\/uniprotkb\/P63039\/entry - NCBI Gene search (HSP60) — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=HSP60 - Ensembl search (HSP60) — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=HSP60 - PubMed search (HSP60) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=HSP60 - Reactome pathway search (HSP60) — Reactome — https:\/\/reactome.org\/content\/query?q=HSP60 --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"0.5mg\/ml if reconstituted with 0.2ml sterile DI water \/ 100 ug","offer_id":53043256557933,"sku":"R30272","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_ce4f9e34-9a92-428a-b3b4-78eb982ddcec.jpg?v=1771934470","url":"https:\/\/www.ebiohippo.com\/products\/hsp60-antibody-hspd1-bha17104010","provider":"BioHippo","version":"1.0","type":"link"}