{"product_id":"hsp70-antibody-heat-shock-protein-70-hspa8-bha17136235","title":"Hsp70 Antibody \/ Heat shock protein 70 \/ HSPA8","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eHsp70 Antibody \/ Heat shock protein 70 \/ HSPA8 is a anti-HSP70 Rabbit antibody Recombinant Rabbit Monoclonal clone 31H87 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow cytometry (FACS) with listed reactivity in Human.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e HSP70\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Recombinant Rabbit Monoclonal, clone 31H87, isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Liquid\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, ICC, IF, IP, FACS\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eHsp70 antibody detects Heat shock cognate protein 70, also known as HSC70, encoded by the HSPA8 gene. Heat shock cognate protein 70 is a constitutively expressed member of the Hsp70 family of molecular chaperones that regulate protein folding, quality control, and degradation. Hsp70 antibody provides researchers with a highly specific reagent for studying stress responses, proteostasis, and disease mechanisms associated with protein misfolding.\u003cbr\u003e\u003cbr\u003eHeat shock cognate protein 70 binds nascent polypeptides, unfolded proteins, and misfolded intermediates in an ATP dependent cycle. Research using Hsp70 antibody has shown that HSC70 cooperates with Hsp40 co chaperones and nucleotide exchange factors to stabilize client proteins, facilitate folding, and prevent aggregation. This activity is essential for maintaining proteome stability during protein synthesis and cellular stress.\u003cbr\u003e\u003cbr\u003eStudies with Hsp70 antibody have revealed roles in intracellular protein transport. Heat shock cognate protein 70 participates in clathrin mediated endocytosis by uncoating clathrin coated vesicles, ensuring efficient trafficking of receptors and cargo. It also contributes to nuclear import, mitochondrial translocation, and protein transport across membranes. These findings highlight the broad role of HSC70 in protein logistics within the cell.\u003cbr\u003e\u003cbr\u003eBeyond folding and trafficking, Heat shock cognate protein 70 is a central regulator of chaperone mediated autophagy. Research using Hsp70 antibody has demonstrated that HSC70 recognizes proteins containing KFERQ like motifs and directs them to lysosomes for degradation. This selective autophagy pathway maintains cellular homeostasis by removing damaged or superfluous proteins. Dysregulation of this pathway contributes to age related disease and neurodegeneration.\u003cbr\u003e\u003cbr\u003eHSC70 also supports cellular survival under stress. Studies with Hsp70 antibody have shown that its levels increase during stress conditions, such as oxidative stress, heat shock, or metabolic imbalance. By stabilizing key signaling and structural proteins, HSC70 allows cells to adapt and recover from damage. Its protective function underscores why Hsp70 family proteins are considered central to stress tolerance mechanisms.\u003cbr\u003e\u003cbr\u003eIn disease contexts, dysregulation of Heat shock cognate protein 70 has been associated with cancer, infection, and neurodegeneration. Research using Hsp70 antibody has revealed that many tumor cells exploit HSC70 to maintain folding capacity under high metabolic stress, supporting uncontrolled growth. Pathogens, including viruses, hijack HSC70 to aid in replication and assembly. Conversely, in neurodegenerative disorders such as Alzheimer disease, Parkinson disease, and Huntington disease, insufficient chaperone activity allows toxic protein aggregates to accumulate. These findings make HSC70 both a biomarker and a potential therapeutic target.\u003cbr\u003e\u003cbr\u003eHsp70 antibody is widely used in western blotting, immunohistochemistry, and immunofluorescence. Western blotting quantifies expression and post translational modifications, immunohistochemistry reveals tissue distribution, and immunofluorescence demonstrates cytoplasmic and vesicular localization. These approaches make Hsp70 antibody indispensable for molecular chaperone research.\u003cbr\u003e\u003cbr\u003eBy providing validated Hsp70 antibody reagents,\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=HSP70 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=HSP70 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=HSP70 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/HSP70 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=HSP70+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg\/ml BSA \/ 100 ul","offer_id":53047322575213,"sku":"FY13333","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_5d7e6ab5-ea7f-422e-9cb7-a699a66d95f1.jpg?v=1772019432","url":"https:\/\/www.ebiohippo.com\/products\/hsp70-antibody-heat-shock-protein-70-hspa8-bha17136235","provider":"BioHippo","version":"1.0","type":"link"}