{"product_id":"hspa6-antibody-heat-shock-70-kda-protein-6-bha17135341","title":"HSPA6 Antibody \/ Heat shock 70 kDa protein 6","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eHSPA6 Antibody \/ Heat shock 70 kDa protein 6 is a anti-HSPA6 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS) with listed reactivity in Human.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e HSPA6\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, FACS\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eHSPA6 antibody detects Heat shock 70 kDa protein 6, a stress-inducible molecular chaperone that belongs to the HSP70 family of heat shock proteins. The UniProt recommended name is Heat shock 70 kDa protein 6 (HSPA6). This protein is rapidly upregulated in response to heat shock, oxidative stress, and heavy metal exposure, where it assists in refolding denatured proteins and protecting cells from proteotoxic damage.\u003cbr\u003e\u003cbr\u003eFunctionally, HSPA6 antibody identifies a 646-amino-acid cytoplasmic and nuclear chaperone that binds unfolded or misfolded polypeptides to prevent aggregation and promote refolding. HSPA6 operates through an ATP-dependent mechanism, alternating between substrate-binding and release cycles coordinated by its nucleotide-binding and substrate-binding domains. Unlike constitutive HSP70 family members, HSPA6 expression is strictly inducible and transient, making it a sensitive marker of acute cellular stress. It cooperates with co-chaperones such as HSP40 and nucleotide exchange factors to restore proteostasis during recovery from stress.\u003cbr\u003e\u003cbr\u003eThe HSPA6 gene is located on chromosome 1q23.3 and is transcriptionally regulated by heat shock factor 1 (HSF1). Expression is strongly induced by thermal stress and other cytotoxic insults in epithelial, neuronal, and endothelial cells. Under normal physiological conditions, HSPA6 levels are low or undetectable, but during stress, it rapidly accumulates in both the cytoplasm and nucleus, where it stabilizes denatured proteins and facilitates their refolding or degradation.\u003cbr\u003e\u003cbr\u003ePathologically, HSPA6 has been implicated in neuroprotection, cardiac stress response, and cancer cell survival. Upregulation in neurons protects against protein misfolding diseases such as Alzheimer's and Parkinson's, while elevated expression in tumors may contribute to resistance against apoptosis and chemotherapy. In cardiac tissues, HSPA6 supports survival under ischemic or oxidative stress conditions. Research using HSPA6 antibody supports studies in proteostasis, cellular stress pathways, and molecular chaperone biology.\u003cbr\u003e\u003cbr\u003eHSPA6 antibody is validated for use in relevant research applications to detect inducible heat shock proteins and examine cellular responses to thermal or oxidative stress.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=HSPA6 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=HSPA6 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=HSPA6 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/HSPA6 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=HSPA6+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047286989165,"sku":"FY12438","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_d4d0d0d4-891d-47bb-8543-dd262a7f819c.jpg?v=1782236985","url":"https:\/\/www.ebiohippo.com\/products\/hspa6-antibody-heat-shock-70-kda-protein-6-bha17135341","provider":"BioHippo","version":"1.0","type":"link"}