{"product_id":"htra4-antibody-serine-protease-htra4-bha17136129","title":"HTRA4 Antibody \/ Serine protease HTRA4","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eHTRA4 Antibody \/ Serine protease HTRA4 is a anti-HTRA4 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e HTRA4\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eHTRA4 antibody detects Serine protease HTRA4, a member of the high-temperature requirement A (HTRA) family of serine proteases involved in protein quality control, stress response, and apoptosis. The UniProt recommended name is Serine protease HTRA4 (HTRA4). This enzyme participates in proteolytic degradation of misfolded or damaged proteins and contributes to cellular stress adaptation, particularly in placental and cardiovascular tissues.\u003cbr\u003e\u003cbr\u003eFunctionally, HTRA4 antibody identifies a 476-amino-acid secreted and intracellular protease that possesses both trypsin-like catalytic activity and PDZ domain-mediated substrate recognition. HTRA4 cleaves misfolded or aggregated proteins to maintain proteostasis and prevent cellular toxicity. It is expressed primarily in the placenta, where it regulates trophoblast differentiation and contributes to placental remodeling. Additionally, HTRA4 can influence mitochondrial homeostasis and apoptosis by degrading components of the mitochondrial protein import machinery during stress conditions.\u003cbr\u003e\u003cbr\u003eThe HTRA4 gene is located on chromosome 8p13.3 and is highly expressed in placental trophoblasts, heart, and lung. Its expression increases during pregnancy and hypoxia, reflecting roles in stress response and tissue remodeling. Like other HTRA proteases, HTRA4 combines chaperone-like and proteolytic activities to ensure proper protein folding and clearance.\u003cbr\u003e\u003cbr\u003ePathologically, elevated HTRA4 levels have been linked to preeclampsia, where excessive proteolytic activity contributes to endothelial dysfunction and vascular injury. Dysregulation may also play a role in cardiac remodeling, pulmonary hypertension, and neurodegenerative disease by altering extracellular matrix integrity and stress signaling. Research using HTRA4 antibody supports studies in placental biology, protease function, and oxidative stress response.\u003cbr\u003e\u003cbr\u003eHTRA4 antibody is validated for western blotting, immunohistochemistry, and ELISA to detect serine proteases involved in protein homeostasis. \u003cbr\u003e\u003cbr\u003eStructurally, Serine protease HTRA4 contains an N-terminal signal peptide, a trypsin-like catalytic domain with the conserved serine-histidine-aspartate triad, and a C-terminal PDZ domain that mediates substrate recognition and oligomerization. These structural features enable substrate-specific proteolysis and regulated activation under stress. This antibody facilitates analysis of HTRA4's function in proteostasis, placental health, and cardiovascular pathology.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=HTRA4 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=HTRA4 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=HTRA4 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/HTRA4 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=HTRA4+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047319167341,"sku":"FY13227","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_06c112c2-ae22-4c21-b7ec-1843ca007a7c.jpg?v=1782237073","url":"https:\/\/www.ebiohippo.com\/products\/htra4-antibody-serine-protease-htra4-bha17136129","provider":"BioHippo","version":"1.0","type":"link"}