{"product_id":"human-8-iso-prostaglandin-f2-alpha-8-iso-pgf2a-elisa-kit-bhe11402111","title":"Human 8-iso prostaglandin F2α (8-iso-PGF2a) ELISA Kit","description":"\u003cstyle\u003e\n.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}\n.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}\n.bhp-desc p{margin:0 0 12px}\n.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}\n.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}\n.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}\n.bhp-desc .bhp-qc-badge{display:inline-block;background:#e6f4ea;color:#1a7a3c;border:1px solid #a8d5b5;border-radius:4px;padding:6px 14px;font-size:14px;font-weight:600}\n.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}\n.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}\n\u003c\/style\u003e\u003cdiv class=\"bhp-desc\"\u003e\n\u003ch2\u003eAssay Principle\u003c\/h2\u003e\n\u003cp\u003e\u003cspan class=\"bhp-cat-pill\"\u003eSandwich ELISA\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eThis kit employs a two-site sandwich ELISA to quantitate 8-iso-PGF2α in human samples. The microplate is pre-coated with anti-8-iso-PGF2α capture antibody. Samples and standards bind the capture antibody; biotinylated detection antibody and Streptavidin-HRP are applied sequentially; TMB substrate generates colorimetric signal at 450 nm proportional to 8-iso-PGF2α concentration. Sensitivity: 15 pg\/mL; range: 31.25–2,000 pg\/mL.\u003c\/p\u003e\n\u003ch2\u003ePerformance Data\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eAssay Type\u003c\/th\u003e\n\u003ctd\u003eSandwich ELISA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSpecies Reactivity\u003c\/th\u003e\n\u003ctd\u003eHuman (Homo sapiens)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTarget Analyte\u003c\/th\u003e\n\u003ctd\u003e8-iso-Prostaglandin F2α (8-iso-PGF2a; isoprostane)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSensitivity (LLOD)\u003c\/th\u003e\n\u003ctd\u003e15 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eDetection Range\u003c\/th\u003e\n\u003ctd\u003e31.25–2,000 pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSample Types\u003c\/th\u003e\n\u003ctd\u003e⚠ Not specified — confirm with manufacturer before use\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePlate Format\u003c\/th\u003e\n\u003ctd\u003e96-well, available as 48T or 96T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eKit Stability\u003c\/th\u003e\n\u003ctd\u003e≤5% activity loss within stated shelf life\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eManual\u003c\/th\u003e\n\u003ctd\u003eAvailable (PDF, see Documents tab)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eStandard Kit Components\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eComponent\u003c\/th\u003e\n\u003cth\u003eDescription\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eMicroplate\u003c\/th\u003e\n\u003ctd\u003e96-well pre-coated with capture antibody (12×8-well strips)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStandards\u003c\/th\u003e\n\u003ctd\u003eRecombinant 8-iso-PGF2a protein for standard curve\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eDetection Antibody\u003c\/th\u003e\n\u003ctd\u003eBiotinylated anti-8-iso-PGF2a detection antibody\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStreptavidin-HRP\u003c\/th\u003e\n\u003ctd\u003eEnzyme conjugate for signal amplification\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTMB Substrate\u003c\/th\u003e\n\u003ctd\u003e3,3′,5,5′-Tetramethylbenzidine chromogenic substrate\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStop Solution\u003c\/th\u003e\n\u003ctd\u003eSulfuric acid stop solution\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eWash Buffer\u003c\/th\u003e\n\u003ctd\u003eConcentrated wash buffer (dilute before use)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSample Diluent\u003c\/th\u003e\n\u003ctd\u003eFor sample preparation and dilution\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eScientific Background\u003c\/h2\u003e\n\u003cp\u003e8-iso-Prostaglandin F2α (8-iso-PGF2α; also 8-epi-PGF2α or isoprostane iPF₂α-III) is produced by non-enzymatic, free radical-mediated peroxidation of arachidonic acid in membrane phospholipids. Unlike enzymatic prostaglandins, isoprostane synthesis is driven directly by reactive oxygen species (ROS) and is independent of COX enzyme activity. 8-iso-PGF2α is widely recognized as one of the most reliable biomarkers of in vivo oxidative stress and lipid peroxidation. Elevated levels are validated in cardiovascular disease, diabetes, Alzheimer's disease, cancer, and acute lung injury. Measurable in plasma, urine, and tissue samples.\u003c\/p\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\u003cp\u003e\u003cstrong\u003eResearch Use Only (RUO).\u003c\/strong\u003e Not for diagnostic or therapeutic applications. TMB substrate and stop solution are corrosive — wear appropriate PPE. Sodium azide may be present — avoid ingestion.\u003c\/p\u003e\u003c\/div\u003e\n\u003ch2\u003eRelated Products\u003c\/h2\u003e\n\u003cp\u003eBrowse additional ELISA kits and immunoassay reagents in the BioHippo catalog.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"Wuhan Abebio Science Co Ltd","offers":[{"title":"48 T","offer_id":53343665226093,"sku":"AE22959HU-48T","price":290.0,"currency_code":"USD","in_stock":true},{"title":"96 T","offer_id":53343665291629,"sku":"AE22959HU-96T","price":540.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/1_8177854f-bf7a-464e-889d-9e46581dee46.jpg?v=1779069806","url":"https:\/\/www.ebiohippo.com\/products\/human-8-iso-prostaglandin-f2-alpha-8-iso-pgf2a-elisa-kit-bhe11402111","provider":"BioHippo","version":"1.0","type":"link"}