{"product_id":"human-acetylcholinesterase-ache-elisa-kit-bhe11402196","title":"Human Acetylcholinesterase (ACHE) ELISA Kit","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}\u003c\/style\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eSandwich ELISA Kit\u003c\/span\u003e\u003ch2\u003eScientific Background\u003c\/h2\u003e\n\u003cp\u003eAcetylcholinesterase is an enzyme that degrades (through its hydrolytic activity) the neurotransmitter acetylcholine, producing choline and an acetate group. It is mainly found at neuromuscular junctions and cholinergic synapses in the central nervous system, where its activity serves to terminate synaptic transmission.\nAChE has a very high catalytic activity mdash; each molecule of AChE degrades about 5000 molecules of acetylcholine per second. The choline produced by the action of AChE is recycled mdash; it is transported, through reuptake, back into nerve terminals where it is used to synthesize new acetylcholine molecules.Acetylcholinesterase is also found on the red blood cell membranes, where it constitutes the Yt blood group antigen.\u003c\/p\u003e\n\u003ch2\u003eAssay Principle\u003c\/h2\u003e\n\u003cp\u003eThis assay employs a two-site sandwich ELISA to quantitate ACHE in samples. An antibody specific for ACHE has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyACHE present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ACHE is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ACHE bound in the initial step. The color development is stopped and the intensity of the color is measured.\u003c\/p\u003e\n\u003ch2\u003ePerformance Data\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eAssay Type\u003c\/th\u003e\n\u003ctd\u003eSandwich ELISA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eDetection Range\u003c\/th\u003e\n\u003ctd\u003e3.12-200 ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSensitivity\u003c\/th\u003e\n\u003ctd\u003e1.21 ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSpecies Reactivity\u003c\/th\u003e\n\u003ctd\u003eHuman (Homo sapiens)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStability\u003c\/th\u003e\n\u003ctd\u003eThe stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. \nThe loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eManufacturer No.\u003c\/th\u003e\n\u003ctd\u003eAE23415HU\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eUniProt #\u003c\/th\u003e\n\u003ctd\u003eP22303\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAlternative Names\u003c\/th\u003e\n\u003ctd\u003eARACHE; N-ACHE; YT; OTTHUMP00000211347|OTTHUMP00000211349|OTTHUMP00000211356|acetylcholinesterase|apoptosis-related acetylcholinesterase\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eAssay Workflow\u003c\/h2\u003e\n\u003cp\u003eThe kit includes the components required for standard-curve quantification (see the kit manual for the component table and validated sample types). Absorbance is read on a standard microplate reader and concentrations are interpolated from the standard curve.\u003c\/p\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\u003cp\u003eFor research use only (RUO). Not for use in diagnostic or therapeutic procedures. Handle all samples and reagents using standard laboratory safety practices.\u003c\/p\u003e\u003c\/div\u003e\n\u003ch2\u003eRelated Products\u003c\/h2\u003e\n\u003cp\u003eExplore additional Human ELISA kits from Wuhan Abebio Science Co Ltd, or contact us for related assays for ACHE research.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"Wuhan Abebio Science Co Ltd","offers":[{"title":"48 T","offer_id":53728124830061,"sku":"AE23415HU-48T","price":371.0,"currency_code":"USD","in_stock":true},{"title":"96 T","offer_id":53728135905645,"sku":"AE23415HU-96T","price":701.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/1_1c26cb18-3ab1-4dee-b2f8-e2d3cc77fe79.jpg?v=1786720694","url":"https:\/\/www.ebiohippo.com\/products\/human-acetylcholinesterase-ache-elisa-kit-bhe11402196","provider":"BioHippo","version":"1.0","type":"link"}