{"product_id":"human-anti-hepatitis-d-virus-hdv-igg-elisa-kit-bhe21400596","title":"Human Anti-Hepatitis D Virus\/HDV IgG ELISA Kit","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}\u003c\/style\u003e\n\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eThe Human Anti-Hepatitis D Virus\/HDV IgG ELISA Kit quantifies human IgG antibodies against Hepatitis D Virus\/HDV in serum and plasma. The 96-well microplate is pre-coated, and the colorimetric signal is read at 450 nm.\u003c\/p\u003e\n\u003cp\u003eValues are expressed relative to the antibody standard supplied with the kit.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eAssay Type\u003c\/th\u003e\n\u003ctd\u003eQuantitative\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eDetection Method\u003c\/th\u003e\n\u003ctd\u003eColorimetric (OD 450 nm)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSample Type\u003c\/th\u003e\n\u003ctd\u003eSerum, Plasma\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSensitivity\u003c\/th\u003e\n\u003ctd\u003e0.25 ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eDetection Range\u003c\/th\u003e\n\u003ctd\u003e1.56 - 100 ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eRecovery\u003c\/th\u003e\n\u003ctd\u003e80-120%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eKit Size\u003c\/th\u003e\n\u003ctd\u003e96 T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eShipping\u003c\/th\u003e\n\u003ctd\u003e2-8 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eKit Components\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth style=\"width:auto\"\u003eComponent\u003c\/th\u003e\n\u003cth style=\"width:auto\"\u003eQuantity\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eELISA Microtiter plate\u003c\/td\u003e\n\u003ctd\u003e96 wells\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHRP Conjugate\u003c\/td\u003e\n\u003ctd\u003e12 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSample Diluent\u003c\/td\u003e\n\u003ctd\u003e12 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e20×Concentrated Wash Buffer\u003c\/td\u003e\n\u003ctd\u003e50 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSubstrate Reagent A\u003c\/td\u003e\n\u003ctd\u003e6 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSubstrate Reagent B\u003c\/td\u003e\n\u003ctd\u003e6 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStop Solution\u003c\/td\u003e\n\u003ctd\u003e6 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePositive Control\u003c\/td\u003e\n\u003ctd\u003e1 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eNegative Control\u003c\/td\u003e\n\u003ctd\u003e1 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePlate Sealer\u003c\/td\u003e\n\u003ctd\u003e3 pieces\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSealed Bag\u003c\/td\u003e\n\u003ctd\u003e1 piece\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eManual\u003c\/td\u003e\n\u003ctd\u003e1 copy\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eAssay Procedure\u003c\/h2\u003e\n\u003cp\u003eBring all reagents and samples to room temperature (25 °C) before use. All the reagents should be mixed thoroughly by gently swirling before pipetting. Avoid foaming. The unused ELISA Microtiter plate should be sealed as soon as possible and stored at 2-8 °C.\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eNumber the sample and control in order, and keep a record of control wells and sample wells. Set 1 well for blank control, 3 wells for negative control and 2 wells for positive control (A blank well is not needed with dual-wavelength reading). Run samples in duplicate.\u003c\/li\u003e\n\u003cli\u003eA) Add 100 µL positive\/negative control to positive\/negative control well, keep the blank control well empty. b) Dilute the tested Serum with Sample Diluent at 1:10 into sample well (add 100 µL of sample diluent and add 10 µL of serum sample), mix fully.\u003c\/li\u003e\n\u003cli\u003eGently tap the plate to mix thoroughly. Cover the ELISA Microtiter plate with sealer. Incubate for 30 min at 37 °C protected from light.\u003c\/li\u003e\n\u003cli\u003eRemove the plate sealer and aspirate the liquid of each well. Repeat the washing procedure for 5 times with Wash Buffer and immerse for 30-60 s each time. Invert the plate and pat it against thick clean absorbent paper (If bubbles exist in the wells, clean tips can be used to prick them).\u003c\/li\u003e\n\u003cli\u003eAdd 100 µL of HRP Conjugate to each well except the blank control well, mix fully.\u003c\/li\u003e\n\u003cli\u003eGently tap the plate to mix thoroughly, cover the ELISA Microtiter plate with sealer. Incubate for 30 minutes at 37 °C protected from light.\u003c\/li\u003e\n\u003cli\u003eRepeat step 4.\u003c\/li\u003e\n\u003cli\u003eAdd 50 µL of Substrate Reagent A and 50 µL of Substrate Reagent B to each well. Gently tap the plate to mix thoroughly. Cover with a new plate sealer. Incubate for 15 min at 37 °C protected from light.\u003c\/li\u003e\n\u003cli\u003eAdd 50 µL of Stop Solution to each well, gently tap the plate to mix thoroughly.\u003c\/li\u003e\n\u003cli\u003eSet the microplate reader wavelength at 450 nm (it is recommended to set the dual wavelength at 450 nm\/630 nm) to detect A value of each well. Blank well is not essential when using dual wavelength 450 nm\/630 nm for detection. Note: Read the results within 10 min.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003eRequired but not supplied: a microplate reader for 450 nm (dual wavelength 450 nm \/ 630 nm recommended), precision pipettes and tips, a 37 °C incubator or water bath, deionized or distilled water, and absorbent paper.\u003c\/p\u003e\n\u003ch2\u003eStorage \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003eShips at 2-8 °C. Store the kit at 2-8 °C and do not freeze. Once opened, keep the plate at 2-8 °C and use it within 1 month. Bring reagents to room temperature before use and do not mix components from different kit lots.\u003c\/div\u003e\n\u003cp\u003eFor Research Use Only. Not for use in diagnostic procedures.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"AtaGenix Laboratories","offers":[{"title":"96 T","offer_id":67980410093933,"sku":"VK175018-96T","price":650.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/abinScience_Kit_Placeholder_Image_a0c6a8ab-8275-4a25-980b-01cf17a1ac29.jpg?v=1794521020","url":"https:\/\/www.ebiohippo.com\/products\/human-anti-hepatitis-d-virus-hdv-igg-elisa-kit-bhe21400596","provider":"BioHippo","version":"1.0","type":"link"}