{"product_id":"human-bid-shrna-lentivirus-bhv19400147","title":"Human BID shRNA Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eBID (BH3 interacting domain death agonist) is a BH3-only member of the BCL-2 protein family that links the extrinsic and intrinsic apoptotic pathways. Following death receptor activation, caspase-8 cleaves BID to generate truncated BID (tBID), which translocates to mitochondria and activates the effectors BAX and BAK. This triggers mitochondrial outer membrane permeabilization, release of cytochrome c, and downstream caspase activation, amplifying the apoptotic signal. By coupling death receptor signaling to the mitochondrial pathway, BID is essential for efficient apoptosis in many cell types. Its regulation influences sensitivity to cell death, and dysregulation contributes to tumorigenesis and therapy resistance.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe Human BID shRNA Lentivirus delivers validated short hairpin RNA targeting BID from a third-generation, self-inactivating lentiviral backbone. shRNA expression is driven by a U6 promoter, with a co-expressed fluorescent reporter (GFP or RFP) and antibiotic selection marker. VSV-G pseudotyping enables broad tropism across primary, suspension, and cryopreserved cells, and each shRNA is validated for at least 70% BID knockdown by a fluorescence-based method.\u003c\/p\u003e\n\u003cp\u003eHigh-titer particles are ultra-purified by PEG precipitation and sucrose gradient centrifugation. A shRNA set option provides a mix of two independent validated shRNAs plus a scrambled control. Applications include loss-of-function studies of death receptor signaling, mitochondrial apoptosis, and apoptotic crosstalk.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis validated shRNA lentivirus targeting BID (NCBI Accession: NM_197966.3) delivers a 19–20 bp shRNA from a third-generation, self-inactivating lentiviral backbone. Expression is driven from a U6 Pol III promoter, with a constitutively expressed fluorescent reporter (GFP, GFP\/Luc, RFP, RFP\/Luc) and antibiotic selection marker (Blasticidin, Puromycin) co-expressed from the same vector. VSV-G pseudotyping enables broad cell tropism, including primary, suspension, and cryopreserved cell types.\u003c\/p\u003e\n\u003cp\u003eKnockdown is validated using a proprietary bicistronic fluorescence assay in which the target mRNA is co-expressed fused to RFP alongside the shRNA-GFP construct. At least 70% reduction in RFP signal in GFP-positive cells confirms on-target activity — a more direct functional readout than transcript-level qPCR. Polyclonal stable lines can be generated by antibiotic selection within 10 days, preserving parental cell heterogeneity compared to single-clone CRISPR approaches.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GFP \/ Puromycin \/ 5x10^6 (sh-mix) + 5x10^6 (scr-mix)","offer_id":53251615818093,"sku":"LSV-0032-SET1","price":1195.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6 (sh-mix) + 5x10^6 (scr-mix)","offer_id":53311253545325,"sku":"LSV-0032-SET2","price":1195.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Puromycin \/ 5x10^6","offer_id":53311253578093,"sku":"LSV-0032-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6","offer_id":53311253610861,"sku":"LSV-0032-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP\/Luc \/ None \/ 2x10^6","offer_id":53311253643629,"sku":"LSV-0032-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP\/Luc \/ None \/ 2x10^6","offer_id":53311253676397,"sku":"LSV-0032-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Blasticidin \/ 5x10^6","offer_id":53311253709165,"sku":"LSV-0032-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Puromycin \/ 5x10^6","offer_id":53311253741933,"sku":"LSV-0032-6S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"None \/ Puromycin \/ 5x10^6","offer_id":53311253774701,"sku":"LSV-0032-7S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"None \/ Blasticidin \/ 5x10^6","offer_id":53311253807469,"sku":"LSV-0032-8S","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/LSV-0032-h-BID-sh-fig1-1.jpg?v=1776934218","url":"https:\/\/www.ebiohippo.com\/products\/human-bid-shrna-lentivirus-bhv19400147","provider":"BioHippo","version":"1.0","type":"link"}