{"product_id":"human-brak-breast-and-kidney-expressed-chemokine-microsample-elisa-kit-bhe15212633","title":"Human BRAK(Breast And Kidney Expressed Chemokine) Microsample ELISA Kit","description":"\u003ch3\u003eScientific background\u003c\/h3\u003e\u003cp\u003e\u003cstrong\u003eBRAK(Breast And Kidney Expressed Chemokine) Microsample\u003c\/strong\u003e is a chemokine-related marker that helps guide immune cell trafficking by forming concentration gradients.\u003c\/p\u003e\u003cp\u003eChemokine profiles are useful for understanding immune recruitment, chemotaxis, and local inflammatory microenvironments across tissues.\u003c\/p\u003e\u003cp\u003eMeasuring chemokines can support mechanistic links between stimuli (infection, cytokines, injury) and observed immune cell composition or migration.\u003c\/p\u003e\u003ch3\u003eWhy it matters\u003c\/h3\u003e\u003cul\u003e\n\u003cli\u003eQuantify \u003cstrong\u003eBRAK(Breast And Kidney Expressed Chemokine) Microsample\u003c\/strong\u003e to compare biological changes across conditions, doses, or time points.\u003c\/li\u003e\n\u003cli\u003eGenerate concentration data from a standard curve to support biomarker and mechanistic studies.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch3\u003eHow the ELISA works\u003c\/h3\u003e\u003cp\u003eDesigned for \u003cstrong\u003eHuman\u003c\/strong\u003e samples, this kit uses a \u003cstrong\u003eThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human BRAK. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human BRAK. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human BRAK, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human BRAK in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/strong\u003e. After binding and washing, signal is converted to concentration using a standard curve.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSample types\u003c\/strong\u003e: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eDetection range\u003c\/strong\u003e: 0.16-10 ng\/mL\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSensitivity\/LoD\u003c\/strong\u003e: 0.05 ng\/mL\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAssay time\u003c\/strong\u003e: 3.5h\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"ELK Biotechnology","offers":[{"title":"96 T","offer_id":52966438044013,"sku":"ELK10362MS-96T","price":595.4,"currency_code":"USD","in_stock":true},{"title":"48 T","offer_id":52966438076781,"sku":"ELK10362MS-48T","price":416.0,"currency_code":"USD","in_stock":true},{"title":"96 T X 5","offer_id":52966438109549,"sku":"ELK10362MS-96TX5","price":2531.1,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/1h1qbq4v21p4717oo1b_bfadc3a0-322f-41e1-b038-82bd74454ac7.jpg?v=1771848689","url":"https:\/\/www.ebiohippo.com\/products\/human-brak-breast-and-kidney-expressed-chemokine-microsample-elisa-kit-bhe15212633","provider":"BioHippo","version":"1.0","type":"link"}