{"product_id":"human-cd14-positive-monocytes-bhc10900049","title":"Human CD14+ Monocytes","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eHuman CD14+ Monocytes are human frozen primary cells from blood used for innate immune signaling, phagocytic response, and inflammatory pathway studies. These cells display looselyadherent, polygonal growth characteristics.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eBiological source:\u003c\/strong\u003e Human (H. sapiens)\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTissue origin:\u003c\/strong\u003e Blood\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth properties:\u003c\/strong\u003e LooselyAdherent, polygonal\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Frozen\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eReported expression\/markers:\u003c\/strong\u003e CD14+\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eDonor history:\u003c\/strong\u003e Normal tissue\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eBiosafety level:\u003c\/strong\u003e II\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eMonocyte- and macrophage-lineage cells coordinate innate immune sensing, cytokine production, phagocytosis, and tissue-specific inflammatory responses.\u003c\/p\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003ePrimary innate immune cell models are widely used to assess activation-state plasticity and cytokine output.\u003c\/li\u003e\n\u003cli\u003eResearchers often study tissue-specific macrophage phenotypes or donor-dependent inflammatory responses.\u003c\/li\u003e\n\u003cli\u003eCo-culture systems help reveal crosstalk with stromal, epithelial, endothelial, or parenchymal cells.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eMeasure inflammatory mediator release and activation markers after stimulation.\u003c\/li\u003e\n\u003cli\u003eUse in phagocytosis-focused or co-culture assays requiring primary innate immune cells.\u003c\/li\u003e\n\u003cli\u003eEvaluate tissue-specific immune crosstalk in organ-relevant model systems.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eProduct-specific data supplied for this listing\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth Conditions:\u003c\/strong\u003e Use of PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. PriGrow X Series Medium (TM4122) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWarranty:\u003c\/strong\u003e abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and\/or catalog description. Such thirty (30) day period is referred to herein as the \"Warranty Period”.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003ePrimary cells can show donor-dependent and passage-dependent variation, so morphology, growth rate, and marker expression should be interpreted in the context of the specific lot and culture history.\u003c\/li\u003e\n\u003cli\u003eAttachment matrix, medium formulation, and gas conditions can materially influence phenotype maintenance and experimental readouts; use the recommended culture system where possible.\u003c\/li\u003e\n\u003cli\u003eCryopreserved recovery conditions matter for viability and downstream behavior. We recommend using serum-free CryoGuard™ Freezing Media (TM078) or, if serum is preferred, Cryopreservation Medium (TM024).\u003c\/li\u003e\n\u003cli\u003eMarker panels should be interpreted together with morphology and functional readouts rather than as a standalone identity measure.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp style=\"display:none\"\u003eSKU:BHC10900049\u003c\/p\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"5x10\u003csup\u003e5\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53195744182637,"sku":"T4122","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/bfkNaEwAH8EIfWa4Vfj29TMFIfZl1fPyDsL2Qpf4.png?v=1775322467","url":"https:\/\/www.ebiohippo.com\/products\/human-cd14-positive-monocytes-bhc10900049","provider":"BioHippo","version":"1.0","type":"link"}