{"product_id":"human-cortical-gabaergic-neurons-2-0-ipsc-derived-bhc18500317","title":"Human Cortical GABAergic Neurons 2.0\n(iPSC-derived)","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eHuman Cortical GABAergic Neurons 2.0\n(iPSC-derived)\u003c\/strong\u003e is a cell model used for research applications where physiologically relevant identity and donor background support interpretation of experimental readouts. Human Neural within the Nervous system.\u003c\/p\u003e\n\u003cp\u003eiXCells Biotechnologies is proud to provide fully differentiated and functional Human Cortical GABAergic Neurons 2.0 (iPSC-derived) which display typical neuronal morphology (Figure 1), and express typical markers of GABAergic neurons, e.g. GAD67, vGAT (Figure 2, 3), when cultured in the GABAergic Neuron Culture Medium (Cat# MD-0122-100ML). Gamma-aminobutyric acid (GABA) release could be measured by ELISA after 7 days maturation (Figure 4). Moreover, Human GABAergic Neurons 2.0 present spontaneous neuron activity when co-cultured with astrocytes (Figure 5), as assessed by Multi-Electrode Array (MEA), and this activity increases with maturation.\u003c\/p\u003e\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003e\n\u003cstrong\u003eCell identity:\u003c\/strong\u003e Neural (iPSC-Derived Cells)\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eSource context:\u003c\/strong\u003e Cortical GABAergic Neurons 2.0; Nervous\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eBiosafety level:\u003c\/strong\u003e BSL-2 (follow your institution’s biosafety program and local regulations)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eProduct-specific elements (such as tissue source, donor background, and cell classification) help frame how results should be interpreted across assays and experimental conditions.\u003c\/p\u003e\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eNeural and glial cell models support studies of neuronal signaling, synaptic biology, neuroinflammation, and cell-type–specific responses to injury or disease-relevant stimuli.\u003c\/p\u003e\u003cp\u003eAcross primary and specialty cell models, experimental outcomes can be influenced by donor heterogeneity, passage history, confluence, and media composition. For interpretation, it is common to validate key markers or functional phenotypes in the user’s assay context and to document culture variables consistently.\u003c\/p\u003e\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003eIncreasing use of primary and specialty cells to improve translational relevance for target biology and phenotypic screening.\u003c\/li\u003e\n  \u003cli\u003eAdoption of 3D culture formats and co-culture systems to better capture tissue microenvironments and cell–cell interactions.\u003c\/li\u003e\n  \u003cli\u003eIntegration of functional readouts with single-cell and multi-omics profiling to connect phenotype with molecular state.\u003c\/li\u003e\n  \u003cli\u003eGrowth of human-relevant neural models (including glial components) to study circuit- and inflammation-linked phenotypes.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003eProfile identity markers by flow cytometry or immunostaining in cultured cells\u003c\/li\u003e\n  \u003cli\u003eQuantify neurite outgrowth and synaptic marker profiles in neural cultures\u003c\/li\u003e\n  \u003cli\u003eQuantify functional responses to defined stimuli relevant to the model system\u003c\/li\u003e\n  \u003cli\u003eCompare baseline phenotype across donors\/conditions using gene expression profiling\u003c\/li\u003e\n  \u003cli\u003eMeasure neuroinflammatory signaling in neuron–glia or microglia-enriched models\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eInterpretation typically focuses on how a perturbation (e.g., cytokine exposure, metabolic stress, genetic manipulation, or compound treatment) shifts marker profiles or functional readouts relative to an appropriate control matched for donor and culture variables.\u003c\/p\u003e\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003eDonor-to-donor heterogeneity can influence baseline phenotype and treatment response; include biological replicates when feasible.\u003c\/li\u003e\n  \u003cli\u003ePassage number, confluence, and media composition can shift gene expression and functional readouts; track and report these variables consistently.\u003c\/li\u003e\n  \u003cli\u003eContamination control (including routine mycoplasma monitoring) supports reproducibility in downstream assays.\u003c\/li\u003e\n  \u003cli\u003eUse appropriate negative\/positive controls for the readout (e.g., unstimulated controls, pathway agonists\/antagonists) to contextualize observed changes.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c!-- Sources (internal):\n- ATCC Animal Cell Culture Guide — ATCC — https:\/\/www.atcc.org\/resources\/culture-guides\/animal-cell-culture-guide\n- Cell Line Authentication — ATCC — https:\/\/www.atcc.org\/resources\/culture-guides\/cell-line-authentication\n- Biosafety in Microbiological and Biomedical Laboratories (BMBL) — U.S. HHS\/CDC\/NIH — https:\/\/www.cdc.gov\/labs\/BMBL.html\n- Mycoplasma contamination in cell culture — NCBI Bookshelf\/PMC — https:\/\/www.ncbi.nlm.nih.gov\/pmc\/\n- Primary cell culture considerations — Nature Methods — https:\/\/www.nature.com\/nmeth\/\n- Good cell culture practice guidelines — OECD\/ECVAM (concept) — https:\/\/www.oecd.org\/\n--\u003e\n\u003cp style=\"display:none\"\u003eSKU:BHC18500317\u003c\/p\u003e","brand":"iXCells Biotechnologies","offers":[{"title":"Cryopreserved \/ 1 million cells\/vial","offer_id":53197818265965,"sku":"40HU-022","price":855.92,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/40hu-022-highly-characterized-iPSC-derived-GABAergic-Neurons-iXCells-1.jpg?v=1775378646","url":"https:\/\/www.ebiohippo.com\/products\/human-cortical-gabaergic-neurons-2-0-ipsc-derived-bhc18500317","provider":"BioHippo","version":"1.0","type":"link"}