{"product_id":"human-cytomegalovirus-hcmv-dna-polymerase-assay-kit-bht16300025","title":"Human Cytomegalovirus (HCMV) DNA Polymerase Assay Kit","description":"\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}.bhp-desc ol,.bhp-desc ul{margin:0 0 12px 20px}\u003c\/style\u003e\u003cdiv class=\"bhp-desc\"\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eDrug Target Assay Kit\u003c\/span\u003e\u003cp\u003eHuman cytomegalovirus (HCMV), also known as human betaherpesvirus 5, belongs to the herpesviruses. Gene transcription relies on the host RNA polymerase, but the virus carries its own DNA polymerase, a key enzyme of the lytic phase of infection.\u003c\/p\u003e\n\u003ch2\u003eKit vs Kit Plus\u003c\/h2\u003e\n\u003cp\u003eThe only difference is the enzyme: HCMV100K has all reagents except the HCMV DNA polymerase, while HCMV100KE also includes 33 µl of 100 x HCMV DNA polymerase. Both are for 100 assays.\u003c\/p\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eKit (enzyme not included), 100 assays (HCMV100K)\u003c\/th\u003e\n\u003ctd\u003e100 assays\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eKit Plus (enzyme included), 100 assays (HCMV100KE)\u003c\/th\u003e\n\u003ctd\u003e100 assays\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eKit Components\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eComponent\u003c\/th\u003e\n\u003cth\u003eKit (enzyme not included), 100 assays (HCMV100K)\u003c\/th\u003e\n\u003cth\u003eKit Plus (enzyme included), 100 assays (HCMV100KE)\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e10 x Buffer\u003c\/td\u003e\n\u003ctd\u003e400 µl\u003c\/td\u003e\n\u003ctd\u003e400 µl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e100 x DNA template\u003c\/td\u003e\n\u003ctd\u003e33 µl\u003c\/td\u003e\n\u003ctd\u003e33 µl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e100 x dNTP\u003c\/td\u003e\n\u003ctd\u003e33 µl\u003c\/td\u003e\n\u003ctd\u003e33 µl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e2 x Dye\u003c\/td\u003e\n\u003ctd\u003e1550 µl\u003c\/td\u003e\n\u003ctd\u003e1550 µl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e50 mM EDTA\u003c\/td\u003e\n\u003ctd\u003e1550 µl\u003c\/td\u003e\n\u003ctd\u003e1550 µl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e100 x HCMV DNA polymerase\u003c\/td\u003e\n\u003ctd\u003eNot included\u003c\/td\u003e\n\u003ctd\u003e33 µl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eAssay Principle\u003c\/h2\u003e\n\u003cp\u003eThe DNA polymerase makes new DNA from a DNA template and dNTPs. A fluorescence dye diluted in EDTA is then added, and the signal at 535 nm (excitation 485 nm) reflects the DNA made, so inhibitors lower the signal.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eAssay Target\u003c\/th\u003e\n\u003ctd\u003eHCMV DNA polymerase activity\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTarget Type\u003c\/th\u003e\n\u003ctd\u003eDNA Polymerase\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eOrganism\u003c\/th\u003e\n\u003ctd\u003eHuman cytomegalovirus\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAssay Type\u003c\/th\u003e\n\u003ctd\u003eEnzyme Activity Assay; Inhibitor Screening Assay\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eDetection Method\u003c\/th\u003e\n\u003ctd\u003eFluorescence\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eDetection Wavelength\u003c\/th\u003e\n\u003ctd\u003eEx 485 nm \/ Em 535 nm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePlate Format\u003c\/th\u003e\n\u003ctd\u003e384-well or 96-well plate\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eInstrument Required\u003c\/th\u003e\n\u003ctd\u003eFluorescence microplate reader\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAssay Time\u003c\/th\u003e\n\u003ctd\u003eAbout 65 min (60 min reaction + 5 min detection)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSize\u003c\/th\u003e\n\u003ctd\u003e100 assays\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003ePlease contact us to confirm storage conditions.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eShipping\u003c\/th\u003e\n\u003ctd\u003eShipped frozen.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eProtocol\u003c\/h2\u003e\n\u003cp\u003eThe manufacturer's protocol describes the procedure and reaction volumes.\u003c\/p\u003e\n\u003cul\u003e\u003cli\u003eHCMV DNA Polymerase Assay (PDF, see Documents): Covers catalog # HCMV100K, HCMV100KE.\u003c\/li\u003e\u003c\/ul\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003eFor Research Use Only (RUO). Not for use in diagnostic or therapeutic procedures. The Human Cytomegalovirus (HCMV) DNA Polymerase Assay Kit is shipped frozen. Follow standard laboratory safety practices when handling the reagents. Please contact us to confirm storage conditions.\u003c\/div\u003e\n\u003c\/div\u003e","brand":"ProFoldin","offers":[{"title":"Kit (enzyme not included)","offer_id":67794876825965,"sku":"HCMV100K","price":494.0,"currency_code":"USD","in_stock":true},{"title":"Kit Plus (enzyme included)","offer_id":67794882363757,"sku":"HCMV100KE","price":829.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/HCMV_assay_fig.jpg?v=1792791969","url":"https:\/\/www.ebiohippo.com\/products\/human-cytomegalovirus-hcmv-dna-polymerase-assay-kit-bht16300025","provider":"BioHippo","version":"1.0","type":"link"}