{"product_id":"human-dermal-fibroblasts-negative-normal-26-49-years-hdf-n1-bhc10923213","title":"Human Dermal Fibroblasts - Normal (26-49 years) (HDF-n1)","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eHuman Dermal Fibroblasts - Normal (26-49 years) (HDF-n1) are primary fibroblast cells isolated from the dermis of normal human abdominal skin via contraception biopsy. These cells play a key role in maintaining skin structure and function through the production of extracellular matrix components, including collagen, elastin, and fibronectin. The product is supplied as frozen cells.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eBiological source:\u003c\/strong\u003e Human (H. sapiens)\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eTissue origin:\u003c\/strong\u003e Skin\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth properties:\u003c\/strong\u003e Adherent, fibroblast\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Frozen\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eDonor history:\u003c\/strong\u003e Female, 30, Caucasian, BMI 28.96\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eBiosafety level:\u003c\/strong\u003e II\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCulture context:\u003c\/strong\u003e For optimal cell culture, we recommend using PriCoat™ T25 Flasks (G299) or coating your preferred vessels with Applied Cell Extracellular Matrix (G422).\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eFibroblasts are stromal cells that synthesize extracellular matrix, respond to mechanical and inflammatory cues, and shape tissue repair programs through paracrine signaling and matrix remodeling.\u003c\/p\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003ePrimary fibroblasts are used to study donor-specific matrix production and activation-state changes in vitro.\u003c\/li\u003e\n\u003cli\u003eResearchers increasingly examine fibroblast heterogeneity across tissues and disease states.\u003c\/li\u003e\n\u003cli\u003eCo-culture systems are commonly used to evaluate stromal effects on epithelial, immune, or tumor-associated phenotypes.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eMeasure matrix production, activation markers, and cytokine responses under defined stimuli.\u003c\/li\u003e\n\u003cli\u003eStudy wound-healing, fibrosis-associated, or stromal support phenotypes in tissue-relevant contexts.\u003c\/li\u003e\n\u003cli\u003eUse in co-culture assays to evaluate paracrine effects on neighboring cell populations.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eProduct-specific data supplied for this listing\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth Conditions:\u003c\/strong\u003e For optimal cell culture, we recommend using PriCoat™ T25 Flasks (G299) or coating your preferred vessels with Applied Cell Extracellular Matrix (G422). Dulbecco's Modified Eagle Medium (DMEM), High Glucose (TM500) + 10% FBS (*Regular) + 2.5 mM L-glutamine (G275) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂. *Do not heat-inactivate\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eWarranty:\u003c\/strong\u003e abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and\/or catalog description. Such thirty (30) day period is referred to herein as the \"Warranty Period”.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003ePrimary cells can show donor-dependent and passage-dependent variation, so morphology, growth rate, and marker expression should be interpreted in the context of the specific lot and culture history.\u003c\/li\u003e\n\u003cli\u003eAttachment matrix, medium formulation, and gas conditions can materially influence phenotype maintenance and experimental readouts; use the recommended culture system where possible.\u003c\/li\u003e\n\u003cli\u003eCryopreserved recovery conditions matter for viability and downstream behavior. We recommend using serum-free CryoGuard™ Freezing Media (TM078) or, if serum is preferred, Cryopreservation Medium (TM024).\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp style=\"display:none\"\u003eSKU:BHC10923213\u003c\/p\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"5x10\u003csup\u003e5\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53195758207341,"sku":"T4302","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/fKT2gfhkwBfW7jBZxrBQpbsziM7pBfWRXGFHSsuC.png?v=1775322491","url":"https:\/\/www.ebiohippo.com\/products\/human-dermal-fibroblasts-negative-normal-26-49-years-hdf-n1-bhc10923213","provider":"BioHippo","version":"1.0","type":"link"}