{"product_id":"human-erk1-2-extracellular-signal-regulated-kinase-1-2-elisa-kit-bhe15209210","title":"Human ERK1\/2(Extracellular Signal Regulated Kinase 1\/2) ELISA Kit","description":"\u003ch3\u003eScientific background\u003c\/h3\u003e\u003cp\u003e\u003cstrong\u003eERK1\/2 (Extracellular Signal Regulated Kinase 1\/2)\u003c\/strong\u003e is associated with intracellular signaling regulation, often within phosphorylation-dependent pathways that control activation, proliferation, or differentiation.\u003c\/p\u003e\u003cp\u003eWhile kinase activity is frequently assessed by phospho-readouts, total protein abundance can also shift with pathway rewiring, feedback, or changes in cell composition.\u003c\/p\u003e\u003cp\u003eProtein quantification can complement phospho-specific assays and help interpret whether signaling changes are driven by abundance, activation state, or both.\u003c\/p\u003e\u003ch3\u003eWhy it matters\u003c\/h3\u003e\u003cul\u003e\n\u003cli\u003eQuantify \u003cstrong\u003eERK1\/2 (Extracellular Signal Regulated Kinase 1\/2)\u003c\/strong\u003e to compare biological changes across conditions, doses, or time points.\u003c\/li\u003e\n\u003cli\u003eGenerate concentration data from a standard curve to support biomarker and mechanistic studies.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch3\u003eHow the ELISA works\u003c\/h3\u003e\u003cp\u003eDesigned for \u003cstrong\u003eHuman\u003c\/strong\u003e samples, this kit uses a \u003cstrong\u003eThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ERK1\/2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ERK1\/2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ERK1\/2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ERK1\/2 in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/strong\u003e. After binding and washing, signal is converted to concentration using a standard curve.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSample types\u003c\/strong\u003e: tissue homogenates, cell lysates and other biological fluids.\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eDetection range\u003c\/strong\u003e: 0.32-20 ng\/mL\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSensitivity\/LoD\u003c\/strong\u003e: 0.12 ng\/mL\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAssay time\u003c\/strong\u003e: 3.5h\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"ELK Biotechnology","offers":[{"title":"96 T","offer_id":52966053314925,"sku":"ELK0372-96T","price":595.4,"currency_code":"USD","in_stock":true},{"title":"48 T","offer_id":52966053347693,"sku":"ELK0372-48T","price":416.0,"currency_code":"USD","in_stock":true},{"title":"96 T X 5","offer_id":52966053380461,"sku":"ELK0372-96TX5","price":2531.1,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/1h1qbq4v21p4717oo1b_81597735-2a1d-4fc5-a6af-f9be55a06ffc.jpg?v=1771846026","url":"https:\/\/www.ebiohippo.com\/products\/human-erk1-2-extracellular-signal-regulated-kinase-1-2-elisa-kit-bhe15209210","provider":"BioHippo","version":"1.0","type":"link"}