{"product_id":"human-ezr-shrna-lentivirus-bhv19400133","title":"Human EZR shRNA Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eEZR encodes ezrin, a member of the ERM (ezrin-radixin-moesin) protein family that links the plasma membrane to the actin cytoskeleton. Ezrin connects membrane proteins to cortical actin, organizing cell shape, surface structures such as microvilli, cell adhesion, and signal transduction. Through this scaffolding role, ezrin contributes to cell motility, polarity, and the assembly of signaling complexes at the cell cortex. Ezrin is frequently overexpressed in cancers, where it promotes invasion and metastasis, and high expression correlates with poor prognosis in tumors such as osteosarcoma and rhabdomyosarcoma. Ezrin is therefore widely studied as a regulator of cytoskeletal dynamics and as a potential therapeutic target in metastatic disease.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe Human EZR shRNA Lentivirus delivers validated short hairpin RNA targeting human ezrin for stable gene knockdown. The shRNA is validated to achieve at least 70% reduction of EZR using a fluorescence-based assay that provides a direct functional readout rather than transcript-level qPCR. The particles are ultra-purified and concentrated to high titer by PEG precipitation and sucrose gradient centrifugation, and efficiently transduce difficult-to-transfect cells, including primary and thawed cultures. A co-expressed fluorescent reporter (GFP or RFP, optionally with luciferase) and antibiotic selection marker (puromycin or blasticidin) enable stable line generation. A shRNA set option supplies a mix of two independent validated shRNAs plus matched scrambled-control lentivirus for loss-of-function studies of cytoskeletal organization and cell migration.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis validated shRNA lentivirus targeting EZR (NCBI Accession: NM_003379) delivers a 19–20 bp shRNA from a third-generation, self-inactivating lentiviral backbone. Expression is driven from a U6 Pol III promoter, with a constitutively expressed fluorescent reporter (GFP, GFP\/Luc, RFP, RFP\/Luc) and antibiotic selection marker (Blasticidin, Puromycin) co-expressed from the same vector. VSV-G pseudotyping enables broad cell tropism, including primary, suspension, and cryopreserved cell types.\u003c\/p\u003e\n\u003cp\u003eKnockdown is validated using a proprietary bicistronic fluorescence assay in which the target mRNA is co-expressed fused to RFP alongside the shRNA-GFP construct. At least 70% reduction in RFP signal in GFP-positive cells confirms on-target activity — a more direct functional readout than transcript-level qPCR. Polyclonal stable lines can be generated by antibiotic selection within 10 days, preserving parental cell heterogeneity compared to single-clone CRISPR approaches.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GFP \/ Puromycin \/ 5x10^6 (sh-mix) + 5x10^6 (scr-mix)","offer_id":53251617948013,"sku":"LSV-0017-SET1","price":1195.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6 (sh-mix) + 5x10^6 (scr-mix)","offer_id":53311331172717,"sku":"LSV-0017-SET2","price":1195.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Puromycin \/ 5x10^6 (sh-mix)","offer_id":53311331205485,"sku":"LSV-0017-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6 (sh-mix)","offer_id":53311331238253,"sku":"LSV-0017-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP\/Luc \/ None \/ 2x10^6 (sh-mix)","offer_id":53311331271021,"sku":"LSV-0017-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP\/Luc \/ None \/ 2x10^6 (sh-mix)","offer_id":53311331303789,"sku":"LSV-0017-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Blasticidin \/ 5x10^6 (sh-mix)","offer_id":53311331336557,"sku":"LSV-0017-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Puromycin \/ 5x10^6 (sh-mix)","offer_id":53311331369325,"sku":"LSV-0017-6S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"None \/ Puromycin \/ 5x10^6 (sh-mix)","offer_id":53311331402093,"sku":"LSV-0017-7S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"None \/ Blasticidin \/ 5x10^6 (sh-mix)","offer_id":53311331434861,"sku":"LSV-0017-8S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP-P2A-FLuc \/ Puromycin \/ 1x10^6 (sh-mix)","offer_id":53311331467629,"sku":"LSV-0017-9S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP-P2A-FLuc \/ Blasticidin \/ 1x10^6 (sh-mix)","offer_id":53311331500397,"sku":"LSV-0017-10S","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/LSV-0017-h-EZR-sh-Fig1.jpg?v=1776934221","url":"https:\/\/www.ebiohippo.com\/products\/human-ezr-shrna-lentivirus-bhv19400133","provider":"BioHippo","version":"1.0","type":"link"}