{"product_id":"human-fcerii-receptor-ii-for-the-fc-region-of-immunoglobulin-e-microsample-elisa-kit-bhe15212325","title":"Human FceRII(Receptor II For The Fc Region Of Immunoglobulin E) Microsample ELISA Kit","description":"\u003ch3\u003eScientific background\u003c\/h3\u003e\u003cp\u003e\u003cstrong\u003eFceRII(Receptor II For The Fc Region Of Immunoglobulin E) Microsample\u003c\/strong\u003e is an immunoglobulin-related marker involved in humoral immunity.\u003c\/p\u003e\u003cp\u003eImmunoglobulin levels are used to evaluate immune status, antibody responses, or background levels in immunology studies and assays.\u003c\/p\u003e\u003cp\u003eQuantitative readouts can support longitudinal monitoring (pre\/post immunization, infection, or therapy) when paired with antigen-specific assays as needed.\u003c\/p\u003e\u003ch3\u003eWhy it matters\u003c\/h3\u003e\u003cul\u003e\n\u003cli\u003eQuantify \u003cstrong\u003eFceRII(Receptor II For The Fc Region Of Immunoglobulin E) Microsample\u003c\/strong\u003e to compare biological changes across conditions, doses, or time points.\u003c\/li\u003e\n\u003cli\u003eGenerate concentration data from a standard curve to support biomarker and mechanistic studies.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch3\u003eHow the ELISA works\u003c\/h3\u003e\u003cp\u003eDesigned for \u003cstrong\u003eHuman\u003c\/strong\u003e samples, this kit uses a \u003cstrong\u003eThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human FceRII. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human FceRII. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human FceRII, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human FceRII in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/strong\u003e. After binding and washing, signal is converted to concentration using a standard curve.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSample types\u003c\/strong\u003e: serum, plasma, tissue homogenates and other biological fluids.\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eDetection range\u003c\/strong\u003e: 0.32-20 ng\/mL\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSensitivity\/LoD\u003c\/strong\u003e: 0.119 ng\/mL\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAssay time\u003c\/strong\u003e: 3.5h\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"ELK Biotechnology","offers":[{"title":"96 T","offer_id":52966407471469,"sku":"ELK1105MS-96T","price":488.8,"currency_code":"USD","in_stock":true},{"title":"48 T","offer_id":52966407504237,"sku":"ELK1105MS-48T","price":344.5,"currency_code":"USD","in_stock":true},{"title":"96 T X 5","offer_id":52966407537005,"sku":"ELK1105MS-96TX5","price":2077.4,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/1h1qbq4v21p4717oo1b_a7f7e3ef-f88c-4a37-bdd2-4ae0dfef22bd.jpg?v=1771848461","url":"https:\/\/www.ebiohippo.com\/products\/human-fcerii-receptor-ii-for-the-fc-region-of-immunoglobulin-e-microsample-elisa-kit-bhe15212325","provider":"BioHippo","version":"1.0","type":"link"}