{"product_id":"human-gla-stable-knockout-hek293-cell-line-bhc10922992","title":"Human GLA Stable Knockout HEK293 Cell Line","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eThis product is a stable CRISPR knockout cell line in which \u003cem\u003eHUMAN GLA\u003c\/em\u003e has been disrupted in the  (Human (\u003cem\u003eH. sapiens\u003c\/em\u003e)) background using CRISPR-Cas9 genome editing. It provides an isogenic model for loss-of-function studies of HUMAN GLA in kidney tissue context. Cells are supplied frozen (1×10⁶ cells \/ 1.0 ml, BSL-II) and grow as adherent, epithelial monolayers.\u003c\/p\u003e\u003ch2\u003eCRISPR Knockout Design\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget gene:\u003c\/strong\u003e \u003cem\u003eHUMAN GLA\u003c\/em\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eKnockout strategy:\u003c\/strong\u003e CRISPR-Cas9–mediated frameshift-inducing INDEL generation; confirmed by sanger sequencing of frameshift-inducing indels\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eDesign notes:\u003c\/strong\u003e This cell line (Cat.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eParental cell line:\u003c\/strong\u003e  — Human (\u003cem\u003eH. sapiens\u003c\/em\u003e), kidney origin\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eGene Background\u003c\/h2\u003e\u003cp\u003e\u003cem\u003eHUMAN GLA\u003c\/em\u003e is a protein-coding gene. Its encoded protein participates in cellular processes relevant to the biological pathways associated with kidney biology. For detailed gene function, pathway context, and disease associations, refer to the \u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=HUMAN%20GLA\" target=\"_blank\"\u003eNCBI Gene\u003c\/a\u003e and \u003ca href=\"https:\/\/www.uniprot.org\/uniprot\/?query=HUMAN%20GLA+Human\" target=\"_blank\"\u003eUniProt\u003c\/a\u003e entries for \u003cem\u003eHUMAN GLA\u003c\/em\u003e.\u003c\/p\u003e\u003ch2\u003eCell Culture Specifications\u003c\/h2\u003e\u003cdl\u003e\n\u003cdt\u003eCulture medium\u003c\/dt\u003e\n\u003cdd\u003ePriCoat™ ECM T25 Flasks (G999) or Applied Cell Extracellular Matrix (G422) are required for cell adhesion to the culture vessels. Dulbecco's Modified Eagle Medium (DMEM), High Glucose (TM500) + 10% FBS(Regular*) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂. *Do not heat-inactivate\u003c\/dd\u003e\n\u003cdt\u003eGrowth properties\u003c\/dt\u003e\n\u003cdd\u003eAdherent, epithelial\u003c\/dd\u003e\n\u003cdt\u003eTissue origin\u003c\/dt\u003e\n\u003cdd\u003eKidney\u003c\/dd\u003e\n\u003cdt\u003eOrganism\u003c\/dt\u003e\n\u003cdd\u003eHuman (\u003cem\u003eH. sapiens\u003c\/em\u003e)\u003c\/dd\u003e\n\u003cdt\u003eBiosafety level\u003c\/dt\u003e\n\u003cdd\u003eBSL-II\u003c\/dd\u003e\n\u003cdt\u003eFormat\u003c\/dt\u003e\n\u003cdd\u003eFrozen; 1×10⁶ cells \/ 1.0 ml\u003c\/dd\u003e\n\u003c\/dl\u003e\u003cp\u003eQuality is assessed by: Confirmed by Sanger Sequencing of frameshift-inducing INDELs.\u003c\/p\u003e\u003ch2\u003eResearch Applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eValidate \u003cem\u003eHUMAN GLA\u003c\/em\u003e knockout by Western blot or qPCR versus the isogenic parental  line.\u003c\/li\u003e\n\u003cli\u003eInvestigate loss-of-function phenotypes: proliferation, viability, morphology, or pathway activity changes.\u003c\/li\u003e\n\u003cli\u003eScreen drug candidates targeting pathways regulated by HUMAN GLA using this KO as an isogenic control.\u003c\/li\u003e\n\u003cli\u003ePerform rescue experiments by re-expressing wild-type \u003cem\u003eHUMAN GLA\u003c\/em\u003e in the knockout background.\u003c\/li\u003e\n\u003cli\u003eConduct transcriptomic or proteomic profiling comparing KO versus parental  cells.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eImportant Notes\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eAdd selection antibiotic to culture medium only after the first passage post-thaw to allow cells to recover.\u003c\/li\u003e\n\u003cli\u003eCell viability upon thaw is warranted for 30 days following shipment when handled per abm guidelines.\u003c\/li\u003e\n\u003cli\u003eFor laboratory research use only. Not intended for diagnostic, therapeutic, or clinical applications.\u003c\/li\u003e\n\u003c\/ul\u003e\u003c!-- Sources (internal):\n- NCBI Gene: HUMAN GLA | NCBI | https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=HUMAN GLA\n- UniProt: HUMAN GLA | UniProt | https:\/\/www.uniprot.org\/uniprot\/?query=HUMAN GLA\n- abm product page | abmgood.com\n--\u003e\n\u003cp style=\"display:none\"\u003eSKU:BHC10922992\u003c\/p\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53192375828845,"sku":"T3398","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/tq4FMyIwtPKyAJU2gA0e076aPcv2UMZDfxoMR4Rq.png?v=1782151801","url":"https:\/\/www.ebiohippo.com\/products\/human-gla-stable-knockout-hek293-cell-line-bhc10922992","provider":"BioHippo","version":"1.0","type":"link"}