{"product_id":"human-glioblastoma-tumor-cell-line-qns140-bhc10902039","title":"Human Glioblastoma Tumor Cell Line (QNS140)","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eThis cell line is non-brain tumor initiating cells (BTIC) cell line and can be used to create novel male and female precilinal models of GBM. This male derived glioblastoma cell line is one of six cell lines isolated from patient tumors.Additional BTIC cell lines available include: Human Glioblastoma Tumor Cell Line (QNS108) (Cat.\u003c\/p\u003e\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eModel identity:\u003c\/strong\u003e Human Glioblastoma Tumor Cell Line (QNS140) is supplied as a tumor cell line derived from Human brain.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth properties:\u003c\/strong\u003e Adherent\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth conditions:\u003c\/strong\u003e Incubate PriCoat™ T25 Flasks (G299) with Poly-O-Ornithine for one hour, wash flasks with PBS(1X) and incubate with laminin coating for a minimum of 1.5 hours. Wash culture vessel 1-2 times with PBS(1X). PriGrow IV (TM004) + 15mM HEPES + 1X Gem21 Neuroplex without Viatmin A Serum Free Supplement (50x stock solution) + 20 ng\/mL hEGF (Z100139) + 20 ng\/mL hFGF2 (Z101456) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂. Thaw cells in T25; detach through gentle pipetting. Do not culture cells with FBS.Note: Cells have a tendency to form hard-to-separate clumps. Although adherent, cells can detach and survive in suspension. Once cells are added to the culture vessel, ensure quick and uniform dispersion of cells to avoid clumping as attachment begins immediately; promptly transfer to incubator under the recommended conditions. Allow cells to recover undisturbed for 48 hours post-thaw.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eProduct format:\u003c\/strong\u003e Frozen, BSL-2\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eThis cell-based model is generally used in neurobiology, differentiation, and cell signaling studies. Donor\/background information is available for contextual interpretation.\u003c\/p\u003e\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eT8150)Human Glioblastoma Tumor Cell Line (QNS120) (Cat. T8151)Human Glioblastoma Tumor Cell Line (QNS140) (Cat T8152)Human Glioblastoma Tumor Cell Line (QNS166) (Cat. T8153)Human Glioblastoma Tumor Cell Line (QNS315) (Cat. T8154)Human Glioblastoma Tumor Cell Line (QNS509) (Cat. T8155) Donor\/background information provided for this product: Glioblastoma.\u003c\/p\u003e\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eCell-line models continue to be used for tumor phenotype comparison, pathway perturbation studies, and assay development in controlled in vitro systems.\u003c\/li\u003e\n\u003cli\u003eEngineered and subtype-defined tumor lines are often used to compare growth behavior, reporter output, and response patterns across matched experimental conditions.\u003c\/li\u003e\n\u003cli\u003eWhen metastatic or lineage features are described, investigators commonly interpret results alongside morphology, passage history, and culture environment.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eNeurobiology-focused studies of growth state, differentiation-associated morphology, and cell signaling changes in culture.\u003c\/li\u003e\n\u003cli\u003eCell-based assays that compare experimental perturbations across defined media and substrate conditions.\u003c\/li\u003e\n\u003cli\u003ePhenotype tracking using morphology, marker expression, or reporter output where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eChanges in morphology, growth rate, viability, or reporter signal are typically interpreted together with passage history, culture matrix, and the specified growth conditions for the model.\u003c\/p\u003e\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eMorphology, doubling behavior, and reporter or marker output can shift with passage number, substrate choice, and medium composition; these variables should be recorded alongside experimental readouts.\u003c\/li\u003e\n\u003cli\u003eMatched controls such as parental cells, untreated cultures, or parallel cultures maintained under identical conditions help distinguish background effects from biology of interest.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCulture and product details\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth Conditions:\u003c\/strong\u003e Incubate PriCoat™ T25 Flasks (G299) with Poly-O-Ornithine for one hour, wash flasks with PBS(1X) and incubate with laminin coating for a minimum of 1.5 hours. Wash culture vessel 1-2 times with PBS(1X). PriGrow IV (TM004) + 15mM HEPES + 1X Gem21 Neuroplex without Viatmin A Serum Free Supplement (50x stock solution) + 20 ng\/mL hEGF (Z100139) + 20 ng\/mL hFGF2 (Z101456) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂. Thaw cells in T25; detach through gentle pipetting. Do not culture cells with FBS.Note: Cells have a tendency to form hard-to-separate clumps. Although adherent, cells can detach and survive in suspension. Once cells are added to the culture vessel, ensure quick and uniform dispersion of cells to avoid clumping as attachment begins immediately; promptly transfer to incubator under the recommended conditions. Allow cells to recover undisturbed for 48 hours post-thaw.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePopulation Doubling Time (h):\u003c\/strong\u003e 136.15\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53180538978669,"sku":"T8152","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/lqnsKcSjhL6w4FNWyfRt4eJ0JV78mqItkqEdphAV.png?v=1774957850","url":"https:\/\/www.ebiohippo.com\/products\/human-glioblastoma-tumor-cell-line-qns140-bhc10902039","provider":"BioHippo","version":"1.0","type":"link"}