{"product_id":"human-glutamatergic-neurons-ipsc-derived-gfp-labeled-normal-bhc18500315","title":"Human Glutamatergic Neurons (iPSC derived, GFP labeled, Normal)","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eHuman Glutamatergic Neurons (iPSC derived, GFP labeled, Normal)\u003c\/strong\u003e is a cell model used for research applications where physiologically relevant identity and donor background support interpretation of experimental readouts. Human Neural within the Nervous system.\u003c\/p\u003e\n\u003cp\u003eThe rapid and highly reproducible generation of mature and functioning neurons from human pluripotent stem cells (hPSCs), neural progenitors, or fibroblasts has been made possible through the utilization of transcription factors. These factors have played a pivotal role in facilitating investigations related to neurodevelopment, disease modeling, drug screening, and neuronal replacement therapies. By employing various combinations of transcription factors along with specific small molecules, researchers have successfully generated populations of glutamatergic neurons [1,2] .This significant breakthrough has provided valuable insights into neural processes and offered potential avenues for therapeutic advancements. This significant breakthrough has provided valuable insights into neural processes and offered potential avenues for therapeutic advancements.iXCells Biotechnologies takes pride in offering fully differentiated and functional human iPSC-derived glutamatergic neurons that exhibit typical neuronal morphology and express key markers associated with glutamatergic identity, such as VGLUT2, MAP2, and TUJ1 (Figure 1A, B). These neurons not only display robust neural activity but also show an increase in activity over time, indicating progressive maturation (Figure 1C, D). When cultured in the Human Glutamatergic Neuron Maturation Media (Cat# MD-0116) , our neurons provide a reliable model for studying neuronal function and responses (Figure 2). Additionally, our iPSC-derived neurons can be co-cultured with glial cells, enabling the development of comprehensive drug screening platforms to evaluate drug efficacy, neurotoxicity, and other neural responses. iXCells Biotechnologies takes pride in offering fully differentiated and functional human iPSC-derived glutamatergic neurons that exhibit typical neuronal morphology and express key markers associated with glutamatergic identity, such as VGLUT2, MAP2, and TUJ1 (Figure 1A, B). These neurons not only display robust neural activity but also show an increase in activity over time, indicating progressive maturation (Figure 1C, D). When cultured in the Human Glutamatergic Neuron Maturation Media (Cat# MD-0116), our neurons provide a reliable model for studying neuronal function and responses (Figure 2). Additionally, our iPSC-derived neurons can be co-cultured with glial cells, enabling the development of comprehensive drug screening platforms to evaluate drug efficacy, neurotoxicity, and other neural responses. Figure 1. Human iPSCs-derived glutamatergic neurons show expression of characteristic biological markers. (A) Immunostaining shows the expression of neuronal makers MAP2 and Tuj1 at 5 days post-thawing. (B) Immunostaining shows expression of glutamatergic marker VGLUT2, 25 days post-thawing Scale bar 30μm. (C) Glutamatergic neurons were differentiated as a monolayer on a MEA plate and co-cultured with iPSC-derived Astrocytes (40HU-008) and subsequently neuronal activity was measured. (D) The electrical parameters were measured in the MEA over time and the results are graphed as mean ± SEM for mean firing rate and number of spikes..\u003c\/p\u003e\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003e\n\u003cstrong\u003eCell identity:\u003c\/strong\u003e Neural (iPSC-Derived Cells)\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eSource context:\u003c\/strong\u003e Glutamatergic Neurons; Nervous\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eBiosafety level:\u003c\/strong\u003e BSL-2 (follow your institution’s biosafety program and local regulations)\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eGrowth properties:\u003c\/strong\u003e Adherent\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eProduct-specific elements (such as tissue source, donor background, and cell classification) help frame how results should be interpreted across assays and experimental conditions.\u003c\/p\u003e\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eNeural and glial cell models support studies of neuronal signaling, synaptic biology, neuroinflammation, and cell-type–specific responses to injury or disease-relevant stimuli.\u003c\/p\u003e\u003cp\u003eAcross primary and specialty cell models, experimental outcomes can be influenced by donor heterogeneity, passage history, confluence, and media composition. For interpretation, it is common to validate key markers or functional phenotypes in the user’s assay context and to document culture variables consistently.\u003c\/p\u003e\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003eIncreasing use of primary and specialty cells to improve translational relevance for target biology and phenotypic screening.\u003c\/li\u003e\n  \u003cli\u003eAdoption of 3D culture formats and co-culture systems to better capture tissue microenvironments and cell–cell interactions.\u003c\/li\u003e\n  \u003cli\u003eIntegration of functional readouts with single-cell and multi-omics profiling to connect phenotype with molecular state.\u003c\/li\u003e\n  \u003cli\u003eGrowth of human-relevant neural models (including glial components) to study circuit- and inflammation-linked phenotypes.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003eProfile identity markers by flow cytometry or immunostaining in cultured cells\u003c\/li\u003e\n  \u003cli\u003eQuantify neurite outgrowth and synaptic marker profiles in neural cultures\u003c\/li\u003e\n  \u003cli\u003eQuantify functional responses to defined stimuli relevant to the model system\u003c\/li\u003e\n  \u003cli\u003eCompare baseline phenotype across donors\/conditions using gene expression profiling\u003c\/li\u003e\n  \u003cli\u003eMeasure neuroinflammatory signaling in neuron–glia or microglia-enriched models\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eInterpretation typically focuses on how a perturbation (e.g., cytokine exposure, metabolic stress, genetic manipulation, or compound treatment) shifts marker profiles or functional readouts relative to an appropriate control matched for donor and culture variables.\u003c\/p\u003e\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003eDonor-to-donor heterogeneity can influence baseline phenotype and treatment response; include biological replicates when feasible.\u003c\/li\u003e\n  \u003cli\u003ePassage number, confluence, and media composition can shift gene expression and functional readouts; track and report these variables consistently.\u003c\/li\u003e\n  \u003cli\u003eContamination control (including routine mycoplasma monitoring) supports reproducibility in downstream assays.\u003c\/li\u003e\n  \u003cli\u003eUse appropriate negative\/positive controls for the readout (e.g., unstimulated controls, pathway agonists\/antagonists) to contextualize observed changes.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c!-- Sources (internal):\n- ATCC Animal Cell Culture Guide — ATCC — https:\/\/www.atcc.org\/resources\/culture-guides\/animal-cell-culture-guide\n- Cell Line Authentication — ATCC — https:\/\/www.atcc.org\/resources\/culture-guides\/cell-line-authentication\n- Biosafety in Microbiological and Biomedical Laboratories (BMBL) — U.S. HHS\/CDC\/NIH — https:\/\/www.cdc.gov\/labs\/BMBL.html\n- Mycoplasma contamination in cell culture — NCBI Bookshelf\/PMC — https:\/\/www.ncbi.nlm.nih.gov\/pmc\/\n- Primary cell culture considerations — Nature Methods — https:\/\/www.nature.com\/nmeth\/\n- Good cell culture practice guidelines — OECD\/ECVAM (concept) — https:\/\/www.oecd.org\/\n--\u003e\n\u003cp style=\"display:none\"\u003eSKU:BHC18500315\u003c\/p\u003e","brand":"iXCells Biotechnologies","offers":[{"title":"Cryopreserved \/ 1 million cells\/vial","offer_id":53197818036589,"sku":"40HU-014-GFP-1M","price":760.24,"currency_code":"USD","in_stock":true},{"title":"Cryopreserved \/ 2 million cells\/vial","offer_id":53197820887405,"sku":"40HU-014-GFP-2M","price":1303.12,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/40HU-014-GFP-1M-optimized.jpg?v=1775378646","url":"https:\/\/www.ebiohippo.com\/products\/human-glutamatergic-neurons-ipsc-derived-gfp-labeled-normal-bhc18500315","provider":"BioHippo","version":"1.0","type":"link"}