{"product_id":"human-groa-cxcl1-growth-regulated-oncogene-alpha-elisa-kit-bhe15204257","title":"Human GROa\/CXCL1(Growth Regulated Oncogene Alpha) ELISA Kit","description":"\u003ch3\u003eScientific background\u003c\/h3\u003e\u003cp\u003e\u003cstrong\u003eGROa\/CXCL1 (Growth Regulated Oncogene Alpha)\u003c\/strong\u003e is a chemokine-related marker that helps guide immune cell trafficking by forming concentration gradients.\u003c\/p\u003e\u003cp\u003eChemokine profiles are useful for understanding immune recruitment, chemotaxis, and local inflammatory microenvironments across tissues.\u003c\/p\u003e\u003cp\u003eMeasuring chemokines can support mechanistic links between stimuli (infection, cytokines, injury) and observed immune cell composition or migration.\u003c\/p\u003e\u003ch3\u003eWhy it matters\u003c\/h3\u003e\u003cul\u003e\n\u003cli\u003eQuantify \u003cstrong\u003eGROa\/CXCL1 (Growth Regulated Oncogene Alpha)\u003c\/strong\u003e to compare biological changes across conditions, doses, or time points.\u003c\/li\u003e\n\u003cli\u003eGenerate concentration data from a standard curve to support biomarker and mechanistic studies.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch3\u003eHow the ELISA works\u003c\/h3\u003e\u003cp\u003eDesigned for \u003cstrong\u003eHuman\u003c\/strong\u003e samples, this kit uses a \u003cstrong\u003eThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human GROa\/CXCL1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GROa\/CXCL1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human GROa\/CXCL1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GROa\/CXCL1 in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/strong\u003e. After binding and washing, signal is converted to concentration using a standard curve.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSample types\u003c\/strong\u003e: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eDetection range\u003c\/strong\u003e: 15.63-1000 pg\/mL\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSensitivity\/LoD\u003c\/strong\u003e: 5.8 pg\/mL\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAssay time\u003c\/strong\u003e: 3.5h\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"ELK Biotechnology","offers":[{"title":"96 T","offer_id":52965430296941,"sku":"ELK1024-96T","price":488.8,"currency_code":"USD","in_stock":true},{"title":"48 T","offer_id":52965430329709,"sku":"ELK1024-48T","price":344.5,"currency_code":"USD","in_stock":true},{"title":"96 T X 5","offer_id":52965430362477,"sku":"ELK1024-96TX5","price":2077.4,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/1h1qbq4v21p4717oo1b_76bdb2b7-35a5-41d8-a24f-685a0884ffbd.jpg?v=1771843380","url":"https:\/\/www.ebiohippo.com\/products\/human-groa-cxcl1-growth-regulated-oncogene-alpha-elisa-kit-bhe15204257","provider":"BioHippo","version":"1.0","type":"link"}