{"product_id":"human-hepatic-sinusoidal-endothelial-cells-hhsec-adult-bhc18500015","title":"Human Hepatic Sinusoidal Endothelial Cells (HHSEC, Adult)","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eHuman Hepatic Sinusoidal Endothelial Cells (HHSEC, Adult)\u003c\/strong\u003e is a cell model used for research applications where physiologically relevant identity and donor background support interpretation of experimental readouts. Human Endothelial Cells derived from Liver (Hepatic Sinusoidal) within the Digestive system.\u003c\/p\u003e\n\u003cp\u003eThe human liver contains two distinct types of endothelial cells: vascular endothelial cells and sinusoidal endothelial cells. Human Hepatic Sinusoidal Endothelial Cells (HHSECs) are a specialized subset of microvascular endothelial cells that exhibit a distinct phenotype compared to other endothelial cell types. HHSECs function as antigen-presenting cells, facilitating antigen presentation to CD4+ T cells, and play a crucial role in regulating the immune response within the liver [1] . Additionally, HHSECs contribute actively to liver repair by serving as dynamic regulators that respond promptly and locally to zonal environmental stimuli [2] . iXCells Biotechnologies provides high quality HHSECs, which are isolated from human liver and cryopreserved at P2, with ≥ 0.5 million cells in each vial. These HHSECs express CD31\/PECAM-1, VE-Cadherin and von Willebrand Factor (vWF). They are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi, and can be further expanded for no more than 3 passages under the conditions suggested by iXCells Biotechnologies. Further expansion may decrease the purity. Figure 1. Phase contrast images of Human Hepatic Sinusoidal Endothelial Cells (HHSEC, Adult). The cells were recovered and seeded at 10,000 cells\/cm2 following iXCells’ protocol. The images were taken at the indicated time post-recovery.\u003c\/p\u003e\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003e\n\u003cstrong\u003eCell identity:\u003c\/strong\u003e Endothelial Cells (Primary Cells)\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eSource context:\u003c\/strong\u003e Liver; Hepatic Sinusoidal; Digestive\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eDonor background:\u003c\/strong\u003e Age: Adult\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eBiosafety level:\u003c\/strong\u003e BSL-2 (follow your institution’s biosafety program and local regulations)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eProduct-specific elements (such as tissue source, donor background, and cell classification) help frame how results should be interpreted across assays and experimental conditions.\u003c\/p\u003e\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eEndothelial cells form the inner lining of blood vessels and regulate barrier function, leukocyte trafficking, coagulation balance, and angiogenic remodeling in response to biomechanical and inflammatory cues.\u003c\/p\u003e\u003cp\u003eAcross primary and specialty cell models, experimental outcomes can be influenced by donor heterogeneity, passage history, confluence, and media composition. For interpretation, it is common to validate key markers or functional phenotypes in the user’s assay context and to document culture variables consistently.\u003c\/p\u003e\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003eIncreasing use of primary and specialty cells to improve translational relevance for target biology and phenotypic screening.\u003c\/li\u003e\n  \u003cli\u003eAdoption of 3D culture formats and co-culture systems to better capture tissue microenvironments and cell–cell interactions.\u003c\/li\u003e\n  \u003cli\u003eIntegration of functional readouts with single-cell and multi-omics profiling to connect phenotype with molecular state.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003eProfile identity markers by flow cytometry or immunostaining in cultured cells\u003c\/li\u003e\n  \u003cli\u003eMeasure barrier function and inflammatory activation in endothelial monolayers\u003c\/li\u003e\n  \u003cli\u003eQuantify functional responses to defined stimuli relevant to the model system\u003c\/li\u003e\n  \u003cli\u003eCompare baseline phenotype across donors\/conditions using gene expression profiling\u003c\/li\u003e\n  \u003cli\u003eAssess adhesion molecule expression and leukocyte interaction under inflammatory cues\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eInterpretation typically focuses on how a perturbation (e.g., cytokine exposure, metabolic stress, genetic manipulation, or compound treatment) shifts marker profiles or functional readouts relative to an appropriate control matched for donor and culture variables.\u003c\/p\u003e\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003eDonor-to-donor heterogeneity can influence baseline phenotype and treatment response; include biological replicates when feasible.\u003c\/li\u003e\n  \u003cli\u003ePassage number, confluence, and media composition can shift gene expression and functional readouts; track and report these variables consistently.\u003c\/li\u003e\n  \u003cli\u003eContamination control (including routine mycoplasma monitoring) supports reproducibility in downstream assays.\u003c\/li\u003e\n  \u003cli\u003eUse appropriate negative\/positive controls for the readout (e.g., unstimulated controls, pathway agonists\/antagonists) to contextualize observed changes.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c!-- Sources (internal):\n- ATCC Animal Cell Culture Guide — ATCC — https:\/\/www.atcc.org\/resources\/culture-guides\/animal-cell-culture-guide\n- Cell Line Authentication — ATCC — https:\/\/www.atcc.org\/resources\/culture-guides\/cell-line-authentication\n- Biosafety in Microbiological and Biomedical Laboratories (BMBL) — U.S. HHS\/CDC\/NIH — https:\/\/www.cdc.gov\/labs\/BMBL.html\n- Mycoplasma contamination in cell culture — NCBI Bookshelf\/PMC — https:\/\/www.ncbi.nlm.nih.gov\/pmc\/\n- Primary cell culture considerations — Nature Methods — https:\/\/www.nature.com\/nmeth\/\n- Good cell culture practice guidelines — OECD\/ECVAM (concept) — https:\/\/www.oecd.org\/\n--\u003e\n\u003cp style=\"display:none\"\u003eSKU:BHC18500015\u003c\/p\u003e","brand":"iXCells Biotechnologies","offers":[{"title":"Cryopreserved \/ 0.5 million cells\/vial","offer_id":53197812269421,"sku":"10HU-021","price":1096.91,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/10HU-021.png?v=1775378646","url":"https:\/\/www.ebiohippo.com\/products\/human-hepatic-sinusoidal-endothelial-cells-hhsec-adult-bhc18500015","provider":"BioHippo","version":"1.0","type":"link"}