{"product_id":"human-hmgb1-high-mobility-group-protein-b1-sandwich-elisa-kit-stje0003057-bhe15503623","title":"Human HMGB1 (High mobility group protein B1) Sandwich ELISA Kit (STJE0003057)","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}\u003c\/style\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eSandwich ELISA Kit\u003c\/span\u003e\u003ch2\u003eAssay Principle\u003c\/h2\u003e\n\u003cp\u003eHMGB1 (high mobility group box 1) is a highly conserved nuclear protein that acts as a chromatin-binding factor and, when released from damaged or activated cells, as a potent damage-associated molecular pattern (DAMP) driving inflammation. Circulating HMGB1 is studied as a biomarker in sepsis, sterile injury, autoimmunity, and cancer.\u003c\/p\u003e\n\u003cp\u003eIn this sandwich-format assay, a plate pre-coated with a capture antibody specific for HMGB1 (High mobility group protein B1) binds analyte from the sample; a biotinylated detection antibody and HRP-conjugate then form the detection complex, and color development is proportional to analyte concentration.\u003c\/p\u003e\n\u003ch2\u003ePerformance Data\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eAssay Type\u003c\/th\u003e\n\u003ctd\u003eSandwich ELISA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAssay Time\u003c\/th\u003e\n\u003ctd\u003e3.5 hours\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eDetection Range\u003c\/th\u003e\n\u003ctd\u003e31.25~2000pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSensitivity\u003c\/th\u003e\n\u003ctd\u003e18.75pg\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eDetection Method\u003c\/th\u003e\n\u003ctd\u003eColorimetric\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSample Types\u003c\/th\u003e\n\u003ctd\u003eSerum, Plasma, Other Biological Fluids\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eReactivity\u003c\/th\u003e\n\u003ctd\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003eIf unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSpecificity\u003c\/th\u003e\n\u003ctd\u003eThis kit recognizes Human HMGB-1 in samples. No significant cross-reactivity or interference between Human HMGB-1 and analogues was observed.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eIntra-assay Precision (CV)\u003c\/th\u003e\n\u003ctd\u003e5.88%, 4.25%, 5.13% (three sample levels)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eInter-assay Precision (CV)\u003c\/th\u003e\n\u003ctd\u003e5.38%, 4.69%, 3.49% (three sample levels)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eRecovery\u003c\/th\u003e\n\u003ctd\u003eSerum 94-108%; EDTA plasma 94-107%; cell culture media 93-105%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eShelf Life\u003c\/th\u003e\n\u003ctd\u003eIndividual components: 6 months when stored separately according to the manual\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eUniProt ID\u003c\/th\u003e\n\u003ctd\u003eHMGB1_HUMAN\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eKit Components\u003c\/h2\u003e\n\u003cp\u003eKit contents as listed by the manufacturer (component quantities shown for 48T \/ 96T where applicable):\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eMicro ELISA Plate (96-well, dismountable)\u003c\/li\u003e\n\u003cli\u003eReference Standard\u003c\/li\u003e\n\u003cli\u003eBiotinylated Detection Antibody (concentrated 100×)\u003c\/li\u003e\n\u003cli\u003eHRP Conjugate (concentrated 100×)\u003c\/li\u003e\n\u003cli\u003eReference Standard \u0026amp; Sample Diluent\u003c\/li\u003e\n\u003cli\u003eBiotinylated Detection Ab Diluent\u003c\/li\u003e\n\u003cli\u003eHRP Conjugate Diluent\u003c\/li\u003e\n\u003cli\u003eWash Buffer (concentrated 25×)\u003c\/li\u003e\n\u003cli\u003eSubstrate Reagent\u003c\/li\u003e\n\u003cli\u003eStop Solution\u003c\/li\u003e\n\u003cli\u003ePlate Sealers\u003c\/li\u003e\n\u003cli\u003eManual and Certificate of Analysis\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eAssay Workflow\u003c\/h2\u003e\n\u003cp\u003eThe kit provides the components required for standard-curve quantification, including pre-coated microplate strips, standards, conjugate, substrate, and wash and stop solutions (see the kit manual for the full component table). Absorbance is read on a standard microplate reader, and analyte concentrations are interpolated from the standard curve. Total hands-on-to-result time is approximately 3.5 hours.\u003c\/p\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\u003cp\u003eFor scientific research use only (RUO). This kit must not be used in diagnostic, therapeutic, or other medical applications. Handle all samples and reagents using standard laboratory safety practices.\u003c\/p\u003e\u003c\/div\u003e\n\u003ch2\u003eRelated Products\u003c\/h2\u003e\n\u003cp\u003eExplore additional Human ELISA kits and matched research antibodies from St John's Laboratory Ltd, or contact us to source related assays for HMGB1 research.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"St John's Laboratory Ltd","offers":[{"title":"24 T","offer_id":53718673457517,"sku":"STJE0003057-24","price":210.0,"currency_code":"USD","in_stock":true},{"title":"48 T","offer_id":53718673654125,"sku":"STJE0003057-48","price":514.0,"currency_code":"USD","in_stock":true},{"title":"96 T","offer_id":53718673686893,"sku":"STJE0003057-96","price":620.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/STJE0003057_1__12167.1648490541.jpg?v=1786551838","url":"https:\/\/www.ebiohippo.com\/products\/human-hmgb1-high-mobility-group-protein-b1-sandwich-elisa-kit-stje0003057-bhe15503623","provider":"BioHippo","version":"1.0","type":"link"}