{"product_id":"human-hs-crp-high-sensitivity-c-reactive-protein-elisa-kit-bhe15210379","title":"Human hs-CRP(high sensitivity C Reactive Protein) ELISA Kit","description":"\u003ch3\u003eScientific background\u003c\/h3\u003e\u003cp\u003e\u003cstrong\u003ehs-CRP (high sensitivity C Reactive Protein)\u003c\/strong\u003e is an acute-phase inflammatory marker that can rise during systemic inflammation, infection, or tissue injury.\u003c\/p\u003e\u003cp\u003eAcute-phase proteins are often used to monitor overall inflammatory burden and compare disease severity across cohorts.\u003c\/p\u003e\u003cp\u003eBecause they integrate signals from multiple inflammatory pathways, they are useful for screening and for tracking response-to-treatment trends over time.\u003c\/p\u003e\u003ch3\u003eWhy it matters\u003c\/h3\u003e\u003cul\u003e\n\u003cli\u003eQuantify \u003cstrong\u003ehs-CRP (high sensitivity C Reactive Protein)\u003c\/strong\u003e to compare biological changes across conditions, doses, or time points.\u003c\/li\u003e\n\u003cli\u003eGenerate concentration data from a standard curve to support biomarker and mechanistic studies.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch3\u003eHow the ELISA works\u003c\/h3\u003e\u003cp\u003eDesigned for \u003cstrong\u003eHuman\u003c\/strong\u003e samples, this kit uses a \u003cstrong\u003eThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human hs-CRP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human hs-CRP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human hs-CRP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human hs-CRP in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/strong\u003e. After binding and washing, signal is converted to concentration using a standard curve.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSample types\u003c\/strong\u003e: serum, plasma, tissue homogenates, cell lysates, urine, cerebrospinal fluid, cell culture supernates and other biological fluids.\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eDetection range\u003c\/strong\u003e: 1.57-100 ng\/mL\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSensitivity\/LoD\u003c\/strong\u003e: 0.5 ng\/mL\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAssay time\u003c\/strong\u003e: 3.5h\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"ELK Biotechnology","offers":[{"title":"96 T","offer_id":52966194446701,"sku":"ELK8534-96T","price":488.8,"currency_code":"USD","in_stock":true},{"title":"48 T","offer_id":52966194479469,"sku":"ELK8534-48T","price":344.5,"currency_code":"USD","in_stock":true},{"title":"96 T X 5","offer_id":52966194512237,"sku":"ELK8534-96TX5","price":2077.4,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/1h1qbq4v21p4717oo1b_fdbae13f-a49a-4f8d-a75b-53ac5b764fa2.jpg?v=1771846603","url":"https:\/\/www.ebiohippo.com\/products\/human-hs-crp-high-sensitivity-c-reactive-protein-elisa-kit-bhe15210379","provider":"BioHippo","version":"1.0","type":"link"}