{"product_id":"human-ifna-br2-interferon-alpha-beta-receptor-2-elisa-kit-bhe15202827","title":"Human IFNa\/bR2(Interferon Alpha\/Beta Receptor 2) ELISA Kit","description":"\u003ch3\u003eScientific background\u003c\/h3\u003e\u003cp\u003e\u003cstrong\u003eIFNa\/bR2 (Interferon Alpha\/Beta Receptor 2)\u003c\/strong\u003e is part of interferon pathway biology that contributes to antiviral defense and immune regulation.\u003c\/p\u003e\u003cp\u003eInterferon signaling can induce broad transcriptional programs; measuring interferon-related proteins helps contextualize pathway strength and timing in vivo and in vitro.\u003c\/p\u003e\u003cp\u003eInterferon markers are often interpreted together with viral load, ISG expression, or immune activation markers to build a coherent response profile.\u003c\/p\u003e\u003ch3\u003eWhy it matters\u003c\/h3\u003e\u003cul\u003e\n\u003cli\u003eQuantify \u003cstrong\u003eIFNa\/bR2 (Interferon Alpha\/Beta Receptor 2)\u003c\/strong\u003e to compare biological changes across conditions, doses, or time points.\u003c\/li\u003e\n\u003cli\u003eGenerate concentration data from a standard curve to support biomarker and mechanistic studies.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch3\u003eHow the ELISA works\u003c\/h3\u003e\u003cp\u003eDesigned for \u003cstrong\u003eHuman\u003c\/strong\u003e samples, this kit uses a \u003cstrong\u003eThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IFNa\/bR2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IFNa\/bR2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IFNa\/bR2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IFNa\/bR2 in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/strong\u003e. After binding and washing, signal is converted to concentration using a standard curve.\u003c\/p\u003e\u003cp\u003e\u003cstrong\u003eSample types\u003c\/strong\u003e: Serum, plasma, urine and other biological fluids.\u003c\/p\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eDetection range\u003c\/strong\u003e: 0.63-40 ng\/mL\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSensitivity\/LoD\u003c\/strong\u003e: 0.25 ng\/mL\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAssay time\u003c\/strong\u003e: 3.5h\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"ELK Biotechnology","offers":[{"title":"96 T","offer_id":52965102748013,"sku":"ELK3549-96T","price":595.4,"currency_code":"USD","in_stock":true},{"title":"48 T","offer_id":52965102780781,"sku":"ELK3549-48T","price":416.0,"currency_code":"USD","in_stock":true},{"title":"96 T X 5","offer_id":52965102813549,"sku":"ELK3549-96TX5","price":2531.1,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/1h1qbq4v21p4717oo1b_ebd37c56-8284-453e-b7ec-5dbbc8d6d42e.jpg?v=1771842550","url":"https:\/\/www.ebiohippo.com\/products\/human-ifna-br2-interferon-alpha-beta-receptor-2-elisa-kit-bhe15202827","provider":"BioHippo","version":"1.0","type":"link"}