{"product_id":"human-intestinal-smooth-muscle-cells-hismc-bhc18500064","title":"Human Intestinal Smooth Muscle Cells (HISMC)","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eHuman Intestinal Smooth Muscle Cells (HISMC)\u003c\/strong\u003e is a cell model used for research applications where physiologically relevant identity and donor background support interpretation of experimental readouts. Human Smooth Muscle Cells derived from Intestine (Intestinal) within the Digestive system.\u003c\/p\u003e\n\u003cp\u003eSmooth muscle contraction is the fundamental event in gastrointestinal motility. Although many biochemical mechanisms in excitation-contraction coupling remain undefined, cytosolic Ca 2+ is recognized as an essential factor in this process. Inflammation of the human intestine leads to increased levels of smooth muscle-specific actin, promoting thickening of the smooth muscle layers. The increased smooth muscle actin may affect force production and further demonstrates the plasticity of smooth muscle cells in the inflamed intestine [1] . Studies also show that human intestinal smooth muscle cells (HISMCs) respond to IL-1β and TNF-α stimulation by releasing IL-6, which may significantly contribute to the overall systemic inflammatory response. [2] . The availability of HISMCs facilitates research into the contractile and proliferative tissue responses of human intestinal smooth muscle. iXCells Biotechnologies provides high-quality HISMCs isolated from human intestine and cryopreserved at P1, with ≥ 0.5 million cells in each vial. These HISMCs express α-smooth muscle actin. They are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi and can be further expanded for no more than 3 passages under the conditions suggested by iXCells Biotechnologies. Further expansion may decrease the purity. Figure 1. Phase contrast images of human Intestinal Smooth Muscle Cells (HISMCs). The cells were recovered and seeded at 10,000 cells\/cm2 following iXCells’ protocol. The images were taken at the indicated time post recovery.\u003c\/p\u003e\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003e\n\u003cstrong\u003eCell identity:\u003c\/strong\u003e Smooth Muscle Cells (Primary Cells)\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eSource context:\u003c\/strong\u003e Intestine; Intestinal; Digestive\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eBiosafety level:\u003c\/strong\u003e BSL-2 (follow your institution’s biosafety program and local regulations)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eProduct-specific elements (such as tissue source, donor background, and cell classification) help frame how results should be interpreted across assays and experimental conditions.\u003c\/p\u003e\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eCells originating from the Digestive system are commonly studied to understand tissue-specific physiology, signaling, and responses to perturbations in controlled in vitro settings.\u003c\/p\u003e\u003cp\u003eAcross primary and specialty cell models, experimental outcomes can be influenced by donor heterogeneity, passage history, confluence, and media composition. For interpretation, it is common to validate key markers or functional phenotypes in the user’s assay context and to document culture variables consistently.\u003c\/p\u003e\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003eIncreasing use of primary and specialty cells to improve translational relevance for target biology and phenotypic screening.\u003c\/li\u003e\n  \u003cli\u003eAdoption of 3D culture formats and co-culture systems to better capture tissue microenvironments and cell–cell interactions.\u003c\/li\u003e\n  \u003cli\u003eIntegration of functional readouts with single-cell and multi-omics profiling to connect phenotype with molecular state.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003eProfile identity markers by flow cytometry or immunostaining in cultured cells\u003c\/li\u003e\n  \u003cli\u003eQuantify functional responses to defined stimuli relevant to the model system\u003c\/li\u003e\n  \u003cli\u003eCompare baseline phenotype across donors\/conditions using gene expression profiling\u003c\/li\u003e\n  \u003cli\u003eModel inflammatory or metabolic stress responses relevant to gastrointestinal tissues\u003c\/li\u003e\n  \u003cli\u003eScreen compounds or genetic perturbations for phenotype modulation using viability or imaging endpoints\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eInterpretation typically focuses on how a perturbation (e.g., cytokine exposure, metabolic stress, genetic manipulation, or compound treatment) shifts marker profiles or functional readouts relative to an appropriate control matched for donor and culture variables.\u003c\/p\u003e\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003eDonor-to-donor heterogeneity can influence baseline phenotype and treatment response; include biological replicates when feasible.\u003c\/li\u003e\n  \u003cli\u003ePassage number, confluence, and media composition can shift gene expression and functional readouts; track and report these variables consistently.\u003c\/li\u003e\n  \u003cli\u003eContamination control (including routine mycoplasma monitoring) supports reproducibility in downstream assays.\u003c\/li\u003e\n  \u003cli\u003eUse appropriate negative\/positive controls for the readout (e.g., unstimulated controls, pathway agonists\/antagonists) to contextualize observed changes.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c!-- Sources (internal):\n- ATCC Animal Cell Culture Guide — ATCC — https:\/\/www.atcc.org\/resources\/culture-guides\/animal-cell-culture-guide\n- Cell Line Authentication — ATCC — https:\/\/www.atcc.org\/resources\/culture-guides\/cell-line-authentication\n- Biosafety in Microbiological and Biomedical Laboratories (BMBL) — U.S. HHS\/CDC\/NIH — https:\/\/www.cdc.gov\/labs\/BMBL.html\n- Mycoplasma contamination in cell culture — NCBI Bookshelf\/PMC — https:\/\/www.ncbi.nlm.nih.gov\/pmc\/\n- Primary cell culture considerations — Nature Methods — https:\/\/www.nature.com\/nmeth\/\n- Good cell culture practice guidelines — OECD\/ECVAM (concept) — https:\/\/www.oecd.org\/\n--\u003e\n\u003cp style=\"display:none\"\u003eSKU:BHC18500064\u003c\/p\u003e","brand":"iXCells Biotechnologies","offers":[{"title":"Cryopreserved \/ 0.5 million cells\/vial","offer_id":53197813711213,"sku":"10HU-201","price":802.33,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/10HU-201.png?v=1782157704","url":"https:\/\/www.ebiohippo.com\/products\/human-intestinal-smooth-muscle-cells-hismc-bhc18500064","provider":"BioHippo","version":"1.0","type":"link"}