{"product_id":"human-keratin-type-i-cytoskeletal-14-krt14-elisa-kit-bhe11405070","title":"Human Keratin, type I cytoskeletal 14 (KRT14) ELISA Kit","description":"\u003cstyle\u003e\n.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}\n.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}\n.bhp-desc p{margin:0 0 12px}\n.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}\n.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}\n.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}\n.bhp-desc .bhp-qc-badge{display:inline-block;background:#e6f4ea;color:#1a7a3c;border:1px solid #a8d5b5;border-radius:4px;padding:6px 14px;font-size:14px;font-weight:600}\n.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}\n.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}\n\u003c\/style\u003e\n\u003cdiv class=\"bhp-desc\"\u003e\n\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eSandwich ELISA Kit\u003c\/span\u003e\n\u003cp\u003eKeratin 14 is a type I cytokeratin. It is usually found as a heterotetramer with two keratin 5 molecules, a type II keratin. Together they form the cytoskeleton of epithelial cells.Mutations in the genes for these keratins are associated with epidermolysis bullosa simplex and Dermatopathia pigmentosa reticularis, both of which are autosomal dominant mutations.\nKRT14 belongs to a large group of acidic type I keratins that interact with basic type II keratins to form the 8-nm cytoskeletal filament…\u003c\/p\u003e\n\u003ch2\u003eAssay Principle\u003c\/h2\u003e\n\u003cp\u003eThis assay employs a two-site sandwich ELISA to quantitate KRT14 in samples. An antibody specific for KRT14 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyKRT14 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KRT14 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avid…\u003c\/p\u003e\n\u003ch2\u003ePerformance Data\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eAssay Type\u003c\/th\u003e\n\u003ctd\u003eSandwich ELISA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eDetection Method\u003c\/th\u003e\n\u003ctd\u003eSandwich ELISA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eDetection Range\u003c\/th\u003e\n\u003ctd\u003e0.156-10 ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\n\u003c\/table\u003e\n\u003ch2\u003eStability \u0026amp; Storage\u003c\/h2\u003e\n\u003cp\u003eThe stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. \nThe loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Bio…\u003c\/p\u003e\n\u003ch2\u003eSafety \u0026amp; Handling\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\n\u003cstrong\u003eFor Research Use Only (RUO).\u003c\/strong\u003e Not intended for diagnostic or therapeutic use. Handle kit reagents according to standard laboratory safety guidelines.\u003c\/div\u003e\n\u003ch2\u003eRelated Products\u003c\/h2\u003e\n\u003cp\u003eBrowse our complete selection of ELISA kits, antibodies, and assay reagents across species at BioHippo.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"Wuhan Abebio Science Co Ltd","offers":[{"title":"48 T","offer_id":53336040309101,"sku":"AE36363HU-48T","price":325.0,"currency_code":"USD","in_stock":true},{"title":"96 T","offer_id":53336048107885,"sku":"AE36363HU-96T","price":610.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/1_1b4adc3c-5733-4945-8e32-e33b82add613.jpg?v=1778915795","url":"https:\/\/www.ebiohippo.com\/products\/human-keratin-type-i-cytoskeletal-14-krt14-elisa-kit-bhe11405070","provider":"BioHippo","version":"1.0","type":"link"}